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  4. Human Delta-like protein 3(DLL3) ELISA kit

Human Delta-like protein 3(DLL3) ELISA kit

说明书下载
  • 中文名称：
    人δ样蛋白3(DLL3)酶联免疫试剂盒
  • 货号：
    CSB-EL006948HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：
    CUSABIO人δ样蛋白3（DLL3）酶联免疫吸附检测试剂盒，采用双抗体夹心法定量检测人血清、血浆或组织匀浆样本中的DLL3蛋白含量，检测灵敏度可达18.75 pg/mL，线性范围为18.75-1200 pg/mL。DLL3是Notch信号通路的关键配体蛋白，在调控细胞分化、增殖及组织发育中发挥重要作用，近年研究发现其在神经内分泌肿瘤、小细胞肺癌等疾病中呈现异常表达特征。本试剂盒通过预包被高特异性捕获抗体，结合样本中的目标蛋白与标记检测抗体形成复合物，经显色反应实现精准定量分析，适用于基础科研中探索DLL3在肿瘤微环境、干细胞分化或疾病生物标志物筛选等方向的作用机制，为体外研究提供可靠的蛋白表达水平数据支持。该试剂盒包含标准品、预包被96孔板及全套实验试剂，操作流程标准化，可满足多种生物样本的高通量检测需求。
  • 别名：
    Delta Drosophila like 3 ELISA Kit; Delta like 3 Drosophila ELISA Kit; Delta like 3 homolog Drosophila ELISA Kit; Delta like 3 protein ELISA Kit; Delta like protein 3 precursor ELISA Kit; Delta-like protein 3 ELISA Kit; Delta3 ELISA Kit; Dll3 ELISA Kit; DLL3_HUMAN ELISA Kit; Drosophila Delta homolog 3 ELISA Kit; SCDO1 ELISA Kit; SCOD1 ELISA Kit; Spondylocostal dysostosis autosomal recessive ELISA Kit
  • 缩写：
    DLL3
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    18.75 pg/mL-1200 pg/mL
  • 灵敏度：
    4.68 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Developmental Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human DLL3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:5Average %105
    Range %100-112
    1:10Average %97
    Range %91-105
    1:20Average %93
    Range %89-100
    1:40Average %95
    Range %90-100
  • 回收率：
    The recovery of human DLL3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8984-93
    EDTA plasma (n=4)9691-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1OD2AverageCorrected
    12002.199 2.354 2.277 2.134
    6001.714 1.811 1.763 1.620
    3001.172 1.104 1.138 0.995
    1500.698 0.674 0.686 0.543
    750.425 0.407 0.416 0.273
    37.50.310 0.329 0.320 0.177
    18.750.207 0.219 0.213 0.070
    00.141 0.145 0.143
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

产品评价

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DLL3 antibodies

DLL3 Proteins

靶点详情

  • 功能：
    Inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm.
  • 基因功能参考文献：
    1. these results reveal that DLL3 is expressed in tumor specimens from most patients with small cell lung cancer PMID: 29290251
    2. Results indicated that DLL3 expression was silenced in hepatocellular carcinoma (HCC) cells by DNA methylation and was more readily affected by histone acetylation than histone methylation (H3K9me2 or H3K27me3). PMID: 29512761
    3. our results indicated epidermal growth factor-like domain multiple 7 protein participates in growth hormone-secreting pituitary adenoma proliferation and invasion regulation via Notch2/DLL3 signaling pathway. These findings raised the possibility that epidermal growth factor-like domain multiple 7 protein might serve as a useful biomarker to assess growth hormone-secreting pituitary adenoma invasion and prognosis PMID: 28705113
    4. The Dll3 was rarely detectable in the para-carcinoma tissues, but positive in 82.1% of non-small cell cancer tissues. PMID: 28007595
    5. Both global haplotype and individual haplotype analyses showed that the haplotypes of SNP1/SNP2/SNP3/SNP4/SNP5 did not correlate with the disease (P >0.05). Together, these data suggest that genetic variants of the DLL3 gene are not associated with CS in the Chinese Han population. PMID: 27472720
    6. DLL3 was silenced by methylation in human human hepatocellular carcinoma and it negatively regulates the growth of human hepatocellular carcinoma cells. PMID: 23337976
    7. We suggest that the three human DLL3 mutations associated with spondylocostal dysplasia are also functionally equivalent to the Dll3(neo) null allele in mice. PMID: 11923214
    8. mutations in DLL3 cause a consistent pattern of abnormal vertebral segmentation in spondylocostal dysostosis PMID: 12746394
    9. no novel or previously described mutations are present in our cohort, indicating that DLL3 mutations may not be a major cause of congenital scoliosis. PMID: 15717203
    10. The intracellular region of Notch ligands Dll1 and Dll3 regulates their trafficking and signaling activity PMID: 18676613

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  • 相关疾病：
    Spondylocostal dysostosis 1, autosomal recessive (SCDO1)
  • 亚细胞定位：
    Membrane; Single-pass type I membrane protein.
  • 数据库链接：

    HGNC: 2909

    OMIM: 277300

    KEGG: hsa:10683

    STRING: 9606.ENSP00000205143

    UniGene: Hs.127792

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