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中文名称：
GFP鼠单克隆抗体
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货号：
CSB-MA000051M1m
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规格：
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图片：
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Western Blot
Positive WB detected in: 50ng recombinant protein
All lanes: GFP antibody at 1:50000, 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000, 1:3200000, 1:6400000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 32 KDa
Observed band size: 32 KDa
Exposure time:5min
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Western Blot
Positive WB detected in: Recombinant protein at 25ng, 12.5ng, 6.25ng, 3.125ng, 1.56ng, 0.78ng, 0.39ng, 0.195ng
All lanes: GFP antibody at 1:2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 32 KDa
Observed band size: 32 KDa
Exposure time:5min
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Western Blot
Positive WB detected in: 1-4 lanes:Recombinant proteins with GFP tag for 50ng; 5 lane: 293F whole cell lysate transfected with GFP for 5μg
All lanes GFP antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size:1,2,3,4 and 5 is 32,32,50,32,32 KDa respectively
Observed band size: 32,32,50,32,32 KDa
Exposure time:1min
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Immunofluorescence staining of 293F cells transfected with GFP with CSB-MA000051M1m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was R-PE-conjugated Goat Anti-Mouse IgG(H+L).
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Two-color flow cytometric analysis showing 293F cells untransfected (Left) or transfected with GFP (Right) stained with CSB-MA000051M1m at 1:200. The cells were fixed in 70% ethanol at 4℃ overnight. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was Alexa Fluor 647 AffiniPure Donkey Anti-Mouse IgG (H+L) at 1/250 dilution for 30min at 4°C.
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Overlay histogram showing 293F cells untransfected (green line) or transfected with GFP (red line) stained with CSB-MA000051M1m at 1:200. The cells were fixed in 70% ethanol at 4℃ overnight. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was Alexa Fluor 647 AffiniPure Donkey Anti-Mouse IgG (H+L) at 1/250 dilution for 30min at 4°C.
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Immunoprecipitating GFP in 293F whole cell lysate transfected with GFP
Lane 1: Mouse control IgG2b instead of CSB-MA000051M1m in 293F whole cell lysate transfected with GFP
Lane 2: CSB-MA000051M1m (4µg) + 293F whole cell lysate transfected with GFP (500µg)
Lane 3: 293F whole cell lysate transfected with GFP (5µg)
For western blotting, the blot was detected with CSB-MA000051M1m at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000
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其他：
产品详情
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产品描述：
本产品为GFP单克隆抗体（CUSABIO货号：CSB-MA000051M1m），专为检测绿色荧光蛋白（GFP）设计。GFP作为分子生物学中广泛应用的荧光标记蛋白，其特异性检测在基因表达示踪、蛋白定位分析及细胞功能研究中具有重要价值。该抗体通过ELISA、Western Blot（WB）、免疫荧光（IF）、流式细胞术（FC）和免疫沉淀（IP）五大实验平台验证，适用于转基因细胞株筛选、重组蛋白表达验证、荧光融合蛋白追踪等科研场景，展现广泛的物种兼容性。其高灵敏度和特异性可精准识别天然GFP及其衍生变体（如EGFP、YFP），在活细胞成像、蛋白质相互作用研究及报告基因系统中表现卓越。作为科研级试剂，本产品采用高纯度杂交瘤技术制备，批次稳定性优异，为荧光标记实验提供可靠工具，助力基因编辑、细胞信号通路及亚细胞结构研究。核心关键词：GFP单克隆抗体、绿色荧光蛋白检测、免疫印迹抗体、流式细胞术试剂、免疫荧光探针、重组蛋白检测。
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基因名：
GFP
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别名：
CFP, eGFP, eYFP, GFP, GFP tag, YFP
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宿主：
Mouse
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反应种属：
N/A
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免疫原：
Recombinant GFP Protein
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标记方式：
Non-conjugated
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克隆类型：
Monoclonal Antibody
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抗体亚型：
IgG2b
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纯化方式：
>95%,Protein A purified
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克隆号：
6C11C11
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浓度：
It differs from different batches. Please contact us to confirm it.
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保存缓冲液：
Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
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产品提供形式：
liquid
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应用范围：
ELISA, WB, IF, FC, IP
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推荐稀释比：
| Application |
Recommended Dilution |
| WB |
1:50000-1:6400000 |
| IF |
1:50-1:200 |
| FC |
1:100-1:300 |
| IP |
1µl-2µl |
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Protocols：
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储存条件：
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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货期：
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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