Your Good Partner in Biology Research

中文简体

CSF2｜ CTLA4｜ DLL3｜ EGFR｜ IL4｜ IL4R｜ LAG3｜ MSTN｜ NECTIN4｜ NT5E｜ SEZ6｜ TM4SF1｜ TNFSF13｜ TPO

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Human Fibrinogen-like protein 1(FGL1) ELISA kit

Human Fibrinogen-like protein 1(FGL1) ELISA kit

文献引用 (10) 评价 (1) 说明书下载
  • 中文名称：
    人纤维蛋白原样蛋白1(FGL1)酶联免疫试剂盒
  • 货号：
    CSB-EL008653HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    人纤维蛋白原样蛋白1(FGL1)酶联免疫试剂盒（CSB-EL008653HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FGL1含量。FGL1是新兴免疫治疗靶点。它可作为LAG-3的配体，在肿瘤免疫逃逸中发挥重要作用。阻断FGL1与LAG-3相互作用，能解除肿瘤微环境免疫抑制，激活T细胞抗肿瘤活性，目前是肿瘤免疫治疗研究热点。试剂盒检测范围为0.156 ng/ml-10 ng/ml.，该产品适用于探索FGL1在肿瘤微环境中的功能机制、肝脏疾病相关生物标志物筛选，以及代谢综合征等基础研究领域，为免疫调节通路研究、疾病模型构建及药物作用靶点验证提供有效工具。实验操作兼容常规ELISA流程，支持科研人员开展体外样本的定量分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    FGL 1 ELISA Kit; Fgl1 ELISA Kit; FGL1_HUMAN ELISA Kit; Fibrinogen like 1 ELISA Kit; Fibrinogen like protein 1 ELISA Kit; Fibrinogen related protein 1 ELISA Kit; Fibrinogen-like protein 1 ELISA Kit; Hepassocin ELISA Kit; Hepatocellular carcinoma related sequence ELISA Kit; Hepatocyte derived fibrinogen related protein 1 ELISA Kit; Hepatocyte-derived fibrinogen-related protein 1 ELISA Kit; HFREP 1 ELISA Kit; HFREP-1 ELISA Kit; HFREP1 ELISA Kit; HP 041 ELISA Kit; HP-041 ELISA Kit; HP041 ELISA Kit; LFIRE 1 ELISA Kit; LFIRE-1 ELISA Kit; LFIRE1 ELISA Kit; Liver fibrinogen related protein 1 ELISA Kit; Liver fibrinogen-related protein 1 ELISA Kit; MFIRE 1 ELISA Kit; MGC108569 ELISA Kit; MGC12455 ELISA Kit; MGC37822 ELISA Kit; OTTHUMP00000122468 ELISA Kit
  • 缩写：
    FGL1
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 ng/ml-10 ng/ml.
  • 灵敏度：
    0.039 ng/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of Human FGL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    89

    Range %

    84-94

    1:2

    Average %

    87

    Range %

    82-93

    1:4

    Average %

    95

    Range %

    89-99

    1:8

    Average %

    98

    Range %

    92-104

  • 回收率：

    The recovery of Human FGL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    93

    88-97

    EDTA plasma (n=4)

    87

    82-92

  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    10

    2.758

    2.645

    2.702

    2.607

    5

    2.107

    2.256

    2.182

    2.087

    2.5

    1.601

    1.524

    1.563

    1.468

    1.25

    0.867

    0.845

    0.856

    0.761

    0.625

    0.475

    0.483

    0.479

    0.384

    0.312

    0.317

    0.304

    0.311

    0.216

    0.156

    0.181

    0.176

    0.179

    0.084

    0

    0.095

    0.094

    0.095

    　

  • 本试剂盒所含材料：
      • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human FGL1 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial Biotin-labeled FGL1 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
      • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
      • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
      • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
      • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
      • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

    显示更多

    收起更多

  • 本试剂盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

    显示更多

    收起更多

  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

显示更多
收起更多

产品评价

平均分:
5.0分 - 1 个评价

样品类型：血清

样品信息：人

稀释比：其他 1：10

产品评价： 我用CSB-EL008653HU检测正常和疾病组血清，原始OD值为0.1-40，两组结果具有显著差异，用WB验证与之结果一致，认为该结果可信。该试剂盒质量好，灵敏度高。

By 魏老师

相关产品

FGL1 antibodies

FGL1 Proteins

相关问答

■ 常见问题解答
Q:

我们想使用你们的FGL1 Elisa Kit CSB-EL008653HU。不幸的是，我们的血浆样本中混入了柠檬酸钠。但是说明书上只提到EDTA或肝素，我是否可以用这个试剂盒检测我们的样本？

A:
原则上，如果试剂不影响蛋白质结构变化(包括蛋白质变性)，则可以使用该试剂。但过酸或过碱的试剂会影响蛋白质。 我们一般是直接制备血浆检测，不使用其他试剂(如乙酸钠)，所以无法判断会影响检测结果。如果您的样品尚未准备好，建议按照试剂盒的说明操作。如果已经准备好了，请安排一次预实验来检查结果。

靶点详情

  • 功能：
    Immune suppressive molecule that inhibits antigen-specific T-cell activation by acting as a major ligand of LAG3. Responsible for LAG3 T-cell inhibitory function. Binds LAG3 independently from MHC class II (MHC-II). Secreted by, and promotes growth of, hepatocytes.
  • 基因功能参考文献：
    1. FGL1 promotes invasion and metastasis of gastric cancer and is associated with poor prognosis. PMID: 29845203
    2. findings highlight the crucial role of HFREP1 in insulin resistance and diabetes, and provide a potential strategy and biomarker for developing therapeutic approaches to combat these diseases. PMID: 27221093
    3. Hepassocin plays an important role in non-alcoholic fatty liver disease and induces hepatic lipid accumulation through an ERK1/2-dependent pathway. PMID: 23792031
    4. Here, the application of small ubiquitin-related modifier (SUMO) fusion technology in combination with four different chaperone teams on the soluble expression of recombinant HPS protein was explored and analyzed. PMID: 24084006
    5. Findings suggest that hepassocin promotes hepatic cell line L02 cells proliferation via an autocrine mechanism and inhibits HCC cells proliferation by an intracrine pathway. PMID: 21618590
    6. data demonstrated that liver-specific gene LFIRE-1/HFREP-1 was frequently downregulated and might possess growth suppressor activity in HCC PMID: 14981537
    7. HFREP-1 in plasma almost completely bound to the fibrin matrix during clot formation PMID: 16996032
    8. The enhancement of FGL1 levels in vitro by IL-6 and its induction after turpentine oil injection suggest that it is an acute phase reactant. PMID: 18039467
    9. HNF1 binding site and HNF1alpha are critical to liver-specific expression of HPS, and down-regulation or loss of HNF1alpha causes, at least in part, the transcriptional down-regulation of HPS in HCC. PMID: 19304666

    收起更多

  • 亚细胞定位：
    Secreted.
  • 组织特异性：
    Under normal conditions, liver-specific.
  • 数据库链接：

    HGNC: 3695

    OMIM: 605776

    KEGG: hsa:2267

    STRING: 9606.ENSP00000221204

    UniGene: Hs.491143

Most popular with customers

产品中心
特别关注


X