Bovine Deoxyribonuclease-1(DNASE1) ELISA kit
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中文名称：
牛脱氧核糖核酸酶1(DNASE1)酶联免疫试剂盒
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货号：
CSB-EL007049BO
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规格：
96T/48T
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价格：
￥4200/￥3000
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其他：
产品详情
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产品描述：
牛脱氧核糖核酸酶1(DNASE1)酶联免疫试剂盒（CSB-EL007049BO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的DNASE1含量。DNASE1是重要的靶点。它是一种核酸酶，可降解DNA。背景上，其在维持细胞内环境稳定等方面有重要意义。研究机制方面，主要聚焦其降解DNA的具体过程、调控因素，以及在相关疾病如自身免疫病中因功能异常对病情发展的影响。试剂盒检测范围为12.5 pg/mL-800 pg/mL，可为科研领域研究牛DNASE1在细胞凋亡机制、炎症反应或免疫调节中的功能提供可靠工具，尤其适用于畜牧病理学、动物免疫学研究中对相关生物标志物的定量分析需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
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别名：
DNASE1 ELISA Kit; DNL1Deoxyribonuclease-1 ELISA Kit; EC 3.1.21.1 ELISA Kit; Deoxyribonuclease I ELISA Kit; DNase I ELISA Kit
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缩写：
DNASE1
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Uniprot No.：
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种属：
Bos taurus (Bovine)
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样本类型：
serum, plasma, tissue homogenates
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检测范围：
12.5 pg/mL-800 pg/mL
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灵敏度：
3.1 pg/mL
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反应时间：
1-5h
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样本体积：
50-100ul
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检测波长：
450 nm
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研究领域：
Epigenetics and Nuclear Signaling
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测定原理：
quantitative
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测定方法：
Sandwich
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精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine DNASE1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:20 | Average % | 85 | | |
| Range % | 80-89 | | |
| 1:40 | Average % | 94 | | |
| Range % | 90-98 | | |
| 1:80 | Average % | 103 | | |
| Range % | 99-109 | | |
| 1:160 | Average % | 97 | | |
| Range % | 92-101 | | |
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回收率：
| The recovery of bovine DNASE1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 88 | 84-92 | | |
| EDTA plasma (n=4) | 93 | 89-100 | | |
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标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
 | pg/ml | OD1 | OD2 | Average | Corrected | | | | 800 | 2.399 | 2.416 | 2.408 | 2.273 | | | | 400 | 2.213 | 2.195 | 2.204 | 2.069 | | | | 200 | 1.813 | 1.741 | 1.777 | 1.642 | | | | 100 | 1.204 | 1.227 | 1.216 | 1.081 | | | | 50 | 0.683 | 0.713 | 0.698 | 0.563 | | | | 25 | 0.400 | 0.392 | 0.396 | 0.261 | | | | 12.5 | 0.292 | 0.284 | 0.288 | 0.153 | | | | 0 | 0.131 | 0.138 | 0.135 | | | |
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数据处理：
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货期：
3-5 working days
靶点详情
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功能：
Serum endocuclease secreted into body fluids by a wide variety of exocrine and endocrine organs. Expressed by non-hematopoietic tissues and preferentially cleaves protein-free DNA. Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization. Together with DNASE1L3, plays a key role in degrading neutrophil extracellular traps (NETs). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation. Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation.
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基因功能参考文献：
- ascorbic acid has the ability to inhibit DNase I, one of the main endonucleases involved in DNA fragmentation during apoptosis. PMID: 29551685
- the practical capability of this assay system was successfully demonstrated by its use to determine DNase I activity in bovine urine. PMID: 27570054
- Specificity in DNA-DNase I interaction is mediated by DNA flexibility, which influences the induced-fit transitions required to form productive complexes. PMID: 19850052
- results indicated that the backbone hydrogen bonding in the antiparallel beta-sheet formed by its N- and C-termini, rather than the side-chain interactions, is crucial for the correct protein folding PMID: 15311926
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亚细胞定位：
Secreted. Zymogen granule. Nucleus envelope.
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蛋白家族：
DNase I family
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数据库链接：
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