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  4. Bovine Heparin-binding growth factor 2(FGF2) ELISA kit

Bovine Heparin-binding growth factor 2(FGF2) ELISA kit

说明书下载
  • 中文名称：
    牛碱性成纤维细胞生长因子/肝素结合生长因子2(FGF2)酶联免疫试剂盒
  • 货号：
    CSB-EL008625BO
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO牛碱性成纤维细胞生长因子/肝素结合生长因子2（FGF2）定量检测试剂盒，采用双抗体夹心ELISA原理设计，适用于检测牛血清、血浆及组织匀浆样本中FGF2的蛋白表达水平。FGF2作为重要的细胞生长调节因子，广泛参与细胞增殖、分化及血管生成等生物学过程，在组织修复与再生研究中具有重要价值。试剂盒检测范围为6.25-400 pg/mL，灵敏度达6.25 pg/mL，包含预包被捕获抗体的96孔板、生物素标记检测抗体及配套标准品、链霉亲和素-HRP等核心组分，通过显色反应实现目标蛋白的定量分析。所有组分均经严格验证，样本预处理方案可有效降低基质干扰，标准品线性相关系数达0.99以上。本试剂盒操作流程标准化，2-8℃保存条件下有效期达12个月，适用于科研领域中对FGF2功能机制的研究，包括但不限于心血管疾病模型、肿瘤微环境分析、创伤愈合过程等研究方向，可为细胞培养上清液、体液样本及组织提取物中的FGF2动态变化提供可靠检测工具。
  • 别名：
    FGF2Fibroblast growth factor 2 ELISA Kit; FGF-2 ELISA Kit; Basic fibroblast growth factor ELISA Kit; bFGF ELISA Kit; Heparin-binding growth factor 2 ELISA Kit; HBGF-2) [Cleaved into: Kidney-derived growth factor] ELISA Kit
  • 缩写：
    FGF2
  • Uniprot No.：
  • 种属：
    Bos taurus (Bovine)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    6.25 pg/mL-400 pg/mL
  • 灵敏度：
    1.56 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine FGF2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 92
    Range % 87-98
    1:2 Average % 97
    Range % 93-102
    1:4 Average % 84
    Range % 81-89
    1:8 Average % 88
    Range % 83-95
  • 回收率：
    The recovery of bovine FGF2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 101 98-104
    EDTA plasma (n=4) 92 89-97
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    400 2.082 2.212 2.147 1.991
    200 1.298 1.302 1.300 1.144
    100 0.826 0.885 0.856 0.700
    50 0.527 0.564 0.546 0.390
    25 0.341 0.344 0.343 0.187
    12.5 0.288 0.298 0.293 0.137
    6.25 0.229 0.232 0.231 0.075
    0 0.152 0.160 0.156 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

产品评价

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FGF2 Proteins

靶点详情

  • 功能：
    Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4. Also acts as an integrin ligand which is required for FGF2 signaling. Binds to integrin ITGAV:ITGB3. Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration. Functions as a potent mitogen in vitro. Can induce angiogenesis. Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation.
  • 亚细胞定位：
    Secreted. Nucleus.
  • 蛋白家族：
    Heparin-binding growth factors family
  • 数据库链接：

    STRING: 9913.ENSBTAP00000007477

    UniGene: Bt.66957

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