Bovine Inhibin beta A chain(INHBA) ELISA kit

产品详情

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO牛抑制素-βA链（INHBA）酶联免疫试剂盒（货号CSB-EL011719BO），基于竞争法原理实现样本中INHBA的精准定量检测。INHBA作为转化生长因子β（TGF-β）超家族成员，在生殖调控、细胞增殖分化及胚胎发育中发挥重要作用，尤其在卵泡发育和类固醇激素合成等生殖生理过程中具有关键调节功能。本试剂盒可检测血清、血浆及组织匀浆样本，检测灵敏度达15 pg/mL，线性范围为15-600 pg/mL，通过预包被特异性抗体实现目标蛋白捕获，样本中的INHBA与酶标记物竞争结合抗体，最终通过显色反应强度反推浓度值。适用于畜牧业中繁殖性能评估、生殖系统疾病模型研究，以及实验室中对INHBA在组织表达水平、激素调控网络的相关机制探索。该试剂盒操作便捷，提供标准化检测流程，实验数据稳定可靠，但需注意本产品仅限科学研究使用。
别名：

INHBAInhibin beta A chain ELISA Kit; Activin beta-A chain ELISA Kit
缩写：

INHBA
Uniprot No.：

P07995
种属：

Bos taurus (Bovine)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15 pg/mL-600 pg/mL
灵敏度：

15 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine INHBA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	91-98
1:2	Average %	94
	Range %	86-103
1:4	Average %	92
	Range %	89-98
1:8	Average %	96
	Range %	91-102

回收率：

The recovery of bovine INHBA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	92-100
EDTA plasma (n=4)	96	91-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
基因功能参考文献：
1. Results confirm the prodevelopmental actions of activin A and indicate that CTGF may also function as an embryokine by regulating the number of ICM cells in the blastocyst and altering gene expression. Low concentrations of HGF were inhibitory to development. PMID: 27185102
2. The expression of the components of the activin-inhibin-follistatin system is altered in bovine cystic ovarian disease. PMID: 25001504
3. Results support a physiological role for granulosa-derived inhibin as an antagonist of bone morphogenetic protein action on thecal androgen synthesis. PMID: 20739376
4. activin AB is a lignd for activin type IB receptor ALK7 and is responsible for activin-mediated secretion of insulin from pancreatic beta cell line, MIN6. PMID: 15196700
亚细胞定位：

Secreted.
蛋白家族：

TGF-beta family
数据库链接：

KEGG: bta:281867

STRING: 9913.ENSBTAP00000003783

UniGene: Bt.12760