Bovine interleukin 4,IL-4 ELISA Kit

产品详情

文献引用 (6) 说明书下载

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO牛白介素4（IL-4）酶联免疫检测试剂盒（货号：CSB-E12898B），采用双抗体夹心法定量检测血清、血浆及组织匀浆样本中IL-4蛋白的表达水平。IL-4作为Th2型细胞因子，在免疫调节中发挥关键作用，包括促进B细胞增殖分化、调控抗体类别转换，并参与过敏反应及抗寄生虫免疫应答。本试剂盒检测范围为12.5-800 pg/mL，灵敏度达5 pg/mL，试剂盒包含预包被捕获抗体的96孔板、生物素标记检测抗体、IL-4标准品、链霉亲和素-HRP及TMB显色液等核心组分，标准品与检测抗体需-20℃保存，其余组分建议4℃储存，开封后保质期为6个月。适用于科研领域探究牛类感染性疾病、过敏反应或炎症过程中IL-4的动态变化，例如通过血清/血浆样本评估疫苗免疫效果，或利用组织匀浆分析特定器官的免疫应答机制，为畜牧养殖中的疾病机制研究和免疫调控提供实验支持。
别名：

IL4Interleukin-4 ELISA Kit; IL-4 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BSF-1 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit
缩写：

IL4
Uniprot No.：

P30367
种属：

Bos taurus (Bovine)
样本类型：

serum, plasma, tissue homogenates
检测范围：

12.5 pg/mL-800 pg/mL
灵敏度：

3.12 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	89-99
1:2	Average %	97
	Range %	92-103
1:4	Average %	90
	Range %	85-94
1:8	Average %	84
	Range %	80-88

回收率：

The recovery of bovine IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	89	84-93

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
基因功能参考文献：
1. These results support pregnancy as type 2 immune response biased, with increases of IFN-gamma occurring after parturition and an increase in IL-4 production before calving. PMID: 23814356
2. IL-4 may play a role in coordinating the adrenal response to inflammatory stress. PMID: 18055157
3. T helper (Th) type 2 cell cytokine IL-4 modulates airway contraction by secreting matrix metalloproteinase-1 from the smooth muscle cells via phosphatidylinositol 3-kinase activation and changing cell-to-matrix interactions. PMID: 18322231
亚细胞定位：

Secreted.
蛋白家族：

IL-4/IL-13 family
数据库链接：

KEGG: bta:280824

STRING: 9913.ENSBTAP00000021215

UniGene: Bt.423