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  4. Bovine lipopolysaccharide binding protein (LBP) ELISA kit

Bovine lipopolysaccharide binding protein (LBP) ELISA kit

文献引用 (2) 说明书下载
  • 中文名称：
    牛脂多糖结合蛋白(LBP)酶联免疫试剂盒
  • 货号：
    CSB-E16370B
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    牛脂多糖结合蛋白(LBP)酶联免疫试剂盒（CSB-E16370B）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LBP含量。LBP即脂多糖结合蛋白，它在人体免疫防御中扮演重要角色，能与脂多糖结合。研究机制方面，LBP可增强单核细胞、巨噬细胞等对脂多糖的识别和反应，激活免疫信号通路，在炎症反应、抗感染等过程发挥作用，对它的研究有助于深入了解免疫机制。试剂盒检测范围为15.6 ng/mL-1000 ng/mL，适用于牛类感染性疾病模型建立、炎症相关机制研究及LPS介导的病理学分析等科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    LBPLipopolysaccharide-binding protein ELISA Kit; LBP ELISA Kit
  • 缩写：
    LBP
  • Uniprot No.：
  • 种属：
    Bos taurus (Bovine)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 ng/mL-1000 ng/mL
  • 灵敏度：
    3.9 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine LBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 84-102  
    1:2 Average % 86  
    Range % 82-95  
    1:4 Average % 98  
    Range % 90-107  
    1:8 Average % 95  
    Range % 90-100  
  • 回收率：
    The recovery of bovine LBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 88-96  
    EDTA plasma (n=4) 95 90-101  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    1000 2.126 2.150 2.138 2.002  
    500 1.611 1.623 1.617 1.481  
    250 1.142 1.158 1.150 1.014  
    125 0.797 0.780 0.789 0.653  
    62.5 0.463 0.451 0.457 0.321  
    31.25 0.340 0.334 0.337 0.201  
    15.6 0.271 0.275 0.273 0.137  
    0 0.135 0.136 0.136 　  
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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LBP Proteins

相关问答

■ 常见问题解答
Q:

I am interested in the product CSB-E16370B and would like to confirm:
Has this kit actually been validated with bovine samples, or is it based off a sequence homology?

A:
Thanks for your inquiry.
We tested bovine serum and plasma samples before and the test value is 15.6-62.5ng/ml.
We suggest you use the kit for the corresponding species to test your samples.
Pls let me know if you have any further questions. Thank you.

靶点详情

  • 功能：
    Plays a role in the innate immune response. Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. Acts as an affinity enhancer for CD14, facilitating its association with LPS. Promotes the release of cytokines in response to bacterial lipopolysaccharide.
  • 基因功能参考文献：
    1. Our study provides an overview of the gene expression profile between wild LBP and mutant LBP on the LPS-induced inflammatory response of bovine mammary epithelial cells , which will lead to further understanding of the potential effects of LBP mutations on bovine mammary glands. PMID: 26813383
    2. The presence of lipopolysaccharide binding protein (LBP) and alpha1-acid glycoprotein (AGP) was demonstrated in all seven adipose tissue depots. Expression of AGP and LBP suggests that they may have roles as local and systemic inflammatory adipokines. PMID: 25542063
    3. One possible anti-inflammatory mechanism can be attributed to the negative role of BRLBP in suppressing TLR4/NF-kappaB activation mediated by LPS. These findings suggested that BRLBP may be a useful agent to treat LPS-induced mastitis PMID: 25700343
    4. Single nucleotide polymorphisms in the lipopolysaccharide-binding protein may hold the secret of susceptibility to clinical mastitis in Chinese Holstein. PMID: 22733492
  • 亚细胞定位：
    Secreted. Cytoplasmic granule membrane.
  • 蛋白家族：
    BPI/LBP/Plunc superfamily, BPI/LBP family
  • 数据库链接：

    KEGG: bta:512242

    STRING: 9913.ENSBTAP00000022428

    UniGene: Bt.24181

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