Bovine transforming growth factors β2,TGFβ2 ELISA Kit
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中文名称：
牛转化生长因子β2(TGFβ2)酶联免疫试剂盒
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货号：
CSB-E14209B
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规格：
96T/48T
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价格：
￥3600/￥2500
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其他：
产品详情
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产品描述：
CUSABIO牛转化生长因子β2（TGFβ2）酶联免疫检测试剂盒（货号CSB-E14209B），基于竞争法原理实现样本中TGFβ2的定量分析，适用于检测血清、血浆及组织匀浆样本，检测灵敏度达6.25 ng/mL，线性范围覆盖6.25-100 ng/mL。TGFβ2是转化生长因子β超家族的重要成员，参与调节细胞增殖、分化及免疫应答等生理过程，尤其在组织修复和胚胎发育中发挥关键作用。CUSABIO试剂盒采用预包被特异性抗体的固相体系，通过样本中的TGFβ2与生物素标记抗原竞争结合抗体，结合链霉亲和素-HRP催化显色反应，实现目标蛋白的精确定量。其高灵敏度与宽泛检测范围可满足多种实验需求，适用于动物模型研究、疾病机制分析（如炎症反应、纤维化或肿瘤微环境研究）以及生物标志物筛选等科研场景，支持从低丰度到高浓度样本的稳定检测。试剂盒操作流程标准化，兼容常规实验室设备，为探索牛源生物样本中TGFβ2的表达调控及其功能研究提供可靠工具。
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别名：
TGFB2 ELISA Kit; Transforming growth factor beta-2 proprotein ELISA Kit; Milk growth factor ELISA Kit; MGF) [Cleaved into: Latency-associated peptide ELISA Kit; LAP); Transforming growth factor beta-2 ELISA Kit; TGF-beta-2)] ELISA Kit
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缩写：
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Uniprot No.：
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种属：
Bos taurus (Bovine)
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样本类型：
serum, plasma, tissue homogenates
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检测范围：
6.25 ng/mL-100 ng/mL
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灵敏度：
6.25 ng/mL
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反应时间：
1-5h
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样本体积：
50-100ul
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检测波长：
450 nm
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研究领域：
Signal Transduction
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测定原理：
quantitative
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测定方法：
Competitive
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精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine TGFβ2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
85 |
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| Range % |
82-89 |
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| 1:2 |
Average % |
83 |
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| Range % |
80-86 |
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| 1:4 |
Average % |
97 |
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| Range % |
93-100 |
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| 1:8 |
Average % |
96 |
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| Range % |
91-101 |
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回收率：
| The recovery of bovine TGFβ2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
89 |
87-93 |
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| EDTA plasma (n=4) |
94 |
89-99 |
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标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
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| 100 |
0.204 |
0.198 |
0.201 |
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| 50 |
0.356 |
0.358 |
0.357 |
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| 25 |
0.592 |
0.572 |
0.582 |
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| 12.5 |
1.107 |
1.105 |
1.106 |
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| 6.25 |
2.332 |
2.369 |
2.351 |
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数据处理：
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货期：
3-5 working days
靶点详情
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功能：
Transforming growth factor beta-2 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains, which constitute the regulatory and active subunit of TGF-beta-2, respectively.; Required to maintain the Transforming growth factor beta-2 (TGF-beta-2) chain in a latent state during storage in extracellular matrix. Associates non-covalently with TGF-beta-2 and regulates its activation via interaction with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2.; Transforming growth factor beta-2: Multifunctional protein that regulates various processes such as angiogenesis and heart development. Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains remain non-covalently linked rendering TGF-beta-2 inactive during storage in extracellular matrix. At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2 and maintain it in a latent state during storage in extracellular milieus. Once activated following release of LAP, TGF-beta-2 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal.
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基因功能参考文献：
- Data show that TGF-beta pathways operate during ovarian fetal development, and fibrillin 3 is highly expressed at a critical stage early in developing human and bovine fetal ovaries. PMID: 21411746
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亚细胞定位：
[Latency-associated peptide]: Secreted, extracellular space, extracellular matrix.; [Transforming growth factor beta-2]: Secreted.
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蛋白家族：
TGF-beta family
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数据库链接：
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