Canine Prostaglandin E2,PG-E2 ELISA Kit

产品详情

文献引用 (2) 说明书下载

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

犬前列腺素E2(PGE2)酶联免疫试剂盒（CSB-E13493c）为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PG-E2含量。PG-E2即前列腺素E2，在人体生理与病理过程中广泛参与。它参与炎症反应、免疫调节等，还与肿瘤微环境形成相关。研究机制上，主要通过与细胞表面受体结合，激活信号通路，影响细胞增殖、凋亡等过程，是疾病治疗研究的潜在靶点。试剂盒检测范围为10 pg/mL-2000 pg/mL，该产品特别适用于研究犬类炎症性疾病（如关节炎、胃肠炎）、免疫应答机制、药物抗炎效果评估，以及生殖系统或肿瘤相关研究中的PGE2动态监测本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

PG-E2
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma, tissue homogenates
检测范围：

10 pg/mL-2000 pg/mL
灵敏度：

5 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine PG-E2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	90-104
1:2	Average %	95
	Range %	89-105
1:4	Average %	93
	Range %	87-100
1:8	Average %	95
	Range %	90-101

回收率：

The recovery of canine PG-E2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	85-96
EDTA plasma (n=4)	96	89-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.