Canine brain natriuretic peptide(BNP)ELISA Kit

产品详情

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO犬脑钠素/脑钠尿肽（BNP）酶联免疫检测试剂盒（货号：CSB-E17436c），采用双抗体夹心法（Sandwich ELISA）实现定量分析，适用于检测犬血清、血浆及组织匀浆样本中的BNP水平。BNP是由心肌细胞分泌的神经激素，在调节体液平衡和心血管功能中起重要作用，其浓度变化常作为评估心功能的重要科研指标。本试剂盒检测范围覆盖12.5-800 pg/mL，通过高特异性抗体对实现精准检测，实验流程包括标准品梯度稀释、样本孵育、显色反应及酶标仪检测，可稳定获取重复性良好的数据。该产品适用于心血管疾病动物模型研究、药物干预效果评估或病理机制探索等科研领域，为研究者提供可靠的BNP浓度检测方案，助力于犬类心脏相关疾病的分子机制解析及生物标志物研究。
别名：

NPPBNatriuretic peptides B ELISA kit; Gamma-brain natriuretic peptide) [Cleaved into: Brain natriuretic peptide 34 ELISA kit; BNP-34); Brain natriuretic peptide 29 ELISA kit; BNP-29)] ELISA kit
缩写：

BNP
Uniprot No.：

P16859
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma, tissue homogenates
检测范围：

12.5 pg/mL-800 pg/mL
灵敏度：

3.12 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine BNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-105
1:2	Average %	91
	Range %	88-96
1:4	Average %	95
	Range %	90-102
1:8	Average %	97
	Range %	92-103

回收率：

The recovery of canine BNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	93-105
EDTA plasma (n=4)	96	92-101

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Cardiac hormone that plays a key role in mediating cardio-renal homeostasis. May also function as a paracrine antifibrotic factor in the heart. Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins that drive various biological responses. Involved in regulating the extracellular fluid volume and maintaining the fluid-electrolyte balance through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Binds the clearance receptor NPR3.; May affect cardio-renal homeostasis. Able to promote the production of cGMP although its potency is very low compared to brain natriuretic peptide 32.
亚细胞定位：

Secreted.; [Brain natriuretic peptide 32]: Secreted.
蛋白家族：

Natriuretic peptide family
组织特异性：

Brain and also in atria, but at much lower levels than ANP.
数据库链接：

STRING: 9615.ENSCAFP00000024316