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  4. Chicken Corticosterone, CORT ELISA kit

Chicken Corticosterone, CORT ELISA kit

文献引用 (16) 说明书下载
  • 中文名称：
    鸡皮质酮(CORT)酶联免疫试剂盒
  • 货号：
    CSB-E11991C
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    鸡皮质酮/肾上腺酮(CORT)酶联免疫试剂盒（CSB-E11991C）为竞争法ELISA试剂盒，定量检测血清、血浆样本中的CORT含量。试剂盒检测范围为0.5 ng/mL-20 ng/mL，广泛应用于禽类应激生物学研究、养殖环境优化评估、神经内分泌调控模型构建等领域，为实验室开展家禽生理状态监测、行为生态学分析及激素代谢通路探索提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 缩写：
    CORT
  • 种属：
    Gallus gallus (Chicken)
  • 样本类型：
    serum, plasma
  • 检测范围：
    0.5 ng/mL-20 ng/mL
  • 灵敏度：
    0.5 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of chicken CORT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 98  
    Range % 94-103  
    1:2 Average % 97  
    Range % 91-101  
    1:4 Average % 90  
    Range % 85-94  
    1:8 Average % 93  
    Range % 87-98  
  • 回收率：
    The recovery of chicken CORT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 84 80-88  
    EDTA plasma (n=4) 95 90-100  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    20 0.161 0.149 0.155    
    10 0.245 0.259 0.252    
    5 0.408 0.394 0.401    
    2 0.658 0.609 0.634    
    1 0.959 0.904 0.932    
    0.5 1.341 1.276 1.309    
    0 1.842 1.772 1.807    
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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相关问答

■ 常见问题解答
Q:

我看到你们有用鸡CORT ELISA试剂盒可以检测EDTA血浆。那么该检测方法是否适用肝素试管收集的血浆呢？

A:
此Elisa试剂盒可以检测血浆，主要用EDTA抗凝剂。肝素血浆理论上可以被检测到，最好是做梯度预实验验证一下。

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