Chicken Immunoglobulin M(IgM)ELISA Kit
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中文名称：
鸡免疫球蛋白M(IgM)酶联免疫试剂盒
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货号：
CSB-E16200C
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规格：
96T/48T
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价格：
￥3800/￥2500
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其他：
产品详情
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产品描述：
鸡免疫球蛋白M(IgM)酶联免疫试剂盒（CSB-E16200C）为竞争法ELISA试剂盒，定量检测血清、血浆样本中的IgM含量。IgM是一种在体液免疫和黏膜免疫中发挥重要作用的免疫球蛋白，具有10或12个抗原结合位点，能够以高亲和力结合抗原靶标。其研究机制涉及IgM五聚体的组装、与黏膜转运受体pIgR的相互作用以及与特异性受体FcμR的结合等。试剂盒检测范围为7.8 ng/mL-2000 ng/mL，科研人员可将其应用于家禽免疫机制研究、疫苗效力评估、传染病模型建立等场景，例如在禽流感疫苗开发中监测免疫鸡群IgM水平变化，或在沙门氏菌感染实验中分析抗体应答动态，为禽类免疫学研究提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
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缩写：
IgM
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种属：
Gallus gallus (Chicken)
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样本类型：
serum, plasma
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检测范围：
7.8 ng/mL-2000 ng/mL
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灵敏度：
7.8 ng/mL
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反应时间：
1-5h
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样本体积：
50-100ul
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检测波长：
450 nm
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研究领域：
Immunology
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测定原理：
quantitative
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测定方法：
Competitive
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精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1000 | Average % | 92 | |
| Range % | 82-96 | |
| 1:2000 | Average % | 94 | |
| Range % | 91-97 | |
| 1:4000 | Average % | 98 | |
| Range % | 92-104 | |
| 1:8000 | Average % | 92 | |
| Range % | 87-98 | |
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回收率：
| The recovery of chicken IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 97 | 91-103 | |
| EDTA plasma (n=4) | 86 | 82-91 | |
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标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | ng/ml | OD1 | OD2 | Average | | | | 2000 | 0.343 | 0.345 | 0.344 | | | | 500 | 0.554 | 0.575 | 0.565 | | | | 125 | 0.827 | 0.856 | 0.842 | | | | 31.25 | 1.122 | 1.158 | 1.140 | | | | 7.81 | 1.267 | 1.367 | 1.317 | | | | 0 | 1.764 | 1.881 | 1.823 | | |
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数据处理：
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货期：
3-5 working days
引用文献
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Dietary incorporation of biological curcumin nanoparticles improved growth performance, ileal architecture, antioxidative status, serum lipid profile, and humoral immune response of heat-stressed broiler chickens
AME Abdel, NM Mesalam, B Yang, MF Elsadek,Poultry Science,2024
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Sustainable Broilers Production Performance under High Stocking Condition through Colocynth Seed Supplementation
FS Nassar,Sustainability,2023
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Influence of Dietary Supplementation of Guava Leaf, Oxytetracycline, and Tert-Butylhydroxytoluene on Growth Performance, Gut Microbial Population, Immune …
KD Adeyemi,Islamic Azad University,2022
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The Preventive Effect and Mechanisms of Adsorbent Supplementation in Low Concentration Aflatoxin B1 Contaminated Diet on Sub-clinical Symptom and Histological Lesions of Broilers
Kun Xie,Poultry Science,2021
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Effects of essential oil/palygorskite composite on performance, egg quality, plasma biochemistry, oxidation status, immune response and intestinal morphology of laying hens
H.Cheng,Poultry Science,2021
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Hydroxy-Selenomethionine Improves the Selenium Status and
Helps to Maintain Broiler Performances under a High Stocking
Density and Heat Stress Conditions through a Better Redox and
Immune Response
H Sun,Antioxidants,2021
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Clostridium butyricum alone or combined with 1, 25‐dihydroxyvitamin D3 improved early‐stage broiler health by modulating intestinal flora
Y Yu,Journal of Applied Microbiology,2021
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Dietary encapsulated Bacillus subtilis and essential oil supplementation improves reproductive performance and hormone concentrations of broiler breeders during the late laying period
Y.B. Chen,Livestock Science,2021
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Effects of different levels of Hermetia illucens larvae meal on performance, egg quality, yolk fatty acid composition and oxidative status of laying hens
Xin Liu,Italian Journal of Animal Science,2021
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Synergistic Effects of Selenium and Zinc Oxide Nanoparticles on Growth Performance, Hemato-biochemical Profile, Immune and Oxidative Stress Responses, and Intestinal Morphometry of Nile Tilapia ( Oreochromis niloticus )
Amany M. Diab,Biological Trace Element Research,2021
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In Ovo and dietary administration of oligosaccharides extracted from palm kernel cake influence general health of pre- and neonatal broiler chicks
Mohammad Faseleh Jahromi.et al,PLoS One,2017
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