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中文名称：
犬肾素(Renin)酶联免疫试剂盒
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货号：
CSB-E08704c
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规格：
96T/48T
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价格：
￥4800/￥3000
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其他：
产品详情
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产品描述：
CUSABIO犬肾素(Renin)酶联免疫定量检测试剂盒（货号：CSB-E08704c），采用双抗体夹心法原理，专为科研领域设计，适用于检测犬血清、血浆及组织匀浆样本中的肾素含量。肾素作为肾素-血管紧张素系统（RAS）的关键调控酶，在血压调节、电解质平衡及体液稳态中发挥重要作用，其定量分析对心血管疾病、肾脏功能及内分泌相关机制研究具有重要意义。试剂盒检测灵敏度高，线性范围覆盖62.5 pg/mL至4000 pg/mL，可精准捕捉样本中微量目标分子，实验操作流程经标准化优化，提供预包被板条与即用型试剂，有效缩短检测时间。该产品适用于实验动物模型（如高血压、肾功能损伤等）中肾素活性变化的动态监测，或用于药物干预、病理生理机制研究中的分子水平分析，为心血管及代谢性疾病的基础科研提供可靠工具。所有组分均经过严格质控，确保批次间稳定性，实验操作需配合酶标仪在450 nm波长下进行数据读取。
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别名：
REN ELISA Kit; Renin ELISA Kit; EC 3.4.23.15 ELISA Kit; Angiotensinogenase ELISA Kit
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缩写：
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Uniprot No.：
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种属：
Canis lupus familiaris (Dog) (Canis familiaris)
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样本类型：
serum, plasma, tissue homogenates
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检测范围：
62.5 pg/mL-4000 pg/mL
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灵敏度：
15.6 pg/mL
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反应时间：
1-5h
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样本体积：
50-100ul
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检测波长：
450 nm
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研究领域：
Cardiovascular
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测定原理：
quantitative
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测定方法：
Sandwich
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精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of dog renin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
93 |
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| Range % |
89-97 |
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| 1:2 |
Average % |
105 |
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| Range % |
100-112 |
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| 1:4 |
Average % |
84 |
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| Range % |
80-88 |
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| 1:8 |
Average % |
95 |
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| Range % |
90-100 |
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回收率：
| The recovery of dog renin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
97 |
90-103 |
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| EDTA plasma (n=4) |
88 |
84-93 |
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标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 4000 |
2.582 |
2.683 |
2.633 |
2.454 |
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| 2000 |
2.204 |
2.104 |
2.154 |
1.975 |
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| 1000 |
1.451 |
1.521 |
1.486 |
1.307 |
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| 500 |
0.864 |
0.908 |
0.886 |
0.707 |
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| 250 |
0.569 |
0.584 |
0.577 |
0.398 |
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| 125 |
0.430 |
0.402 |
0.416 |
0.237 |
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| 62.5 |
0.279 |
0.259 |
0.269 |
0.090 |
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| 0 |
0.182 |
0.176 |
0.179 |
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数据处理：
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货期：
3-5 working days
靶点详情
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功能：
Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
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基因功能参考文献：
- With a moderate deficit in total body sodium and total body water, the renin-angiotensin-aldosterone system is the major factor controlling arterial blood pressure. PMID: 19930429
- Characterization of canine renin with mutations in the substrate binding site. PMID: 17942478
- Normotensive sodium loading in conscious dogs: regulation of renin secretion during beta1-receptor blockade. PMID: 19073902
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亚细胞定位：
Secreted. Membrane.
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蛋白家族：
Peptidase A1 family
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数据库链接：
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