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  4. Dog interleukin 4,IL-4 ELISA Kit

Dog interleukin 4,IL-4 ELISA Kit

文献引用 (1) 说明书下载
  • 中文名称：
    犬白介素4(IL-4)酶联免疫试剂盒
  • 货号：
    CSB-E11259c
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO犬白介素4（IL-4）酶联免疫检测试剂盒（货号：CSB-E11259c），采用双抗体夹心法定量检测犬血清、血浆及组织匀浆样本中的IL-4蛋白浓度。IL-4是Th2细胞分泌的关键细胞因子，在调节体液免疫、过敏反应及抗寄生虫感染中发挥重要作用。本试剂盒检测灵敏度高，线性范围为6.25-400 pg/mL，最低检测限为6.25 pg/mL，操作流程包含标准品梯度稀释、样本孵育、酶标抗体结合及显色反应，可在4.5小时内完成检测。试剂盒包含预包被捕获抗体的96孔板、生物素标记检测抗体、标准品、显色底物及终止液等核心组分，适用于科研领域中对犬类免疫相关疾病机制的研究，例如过敏性疾病模型构建、寄生虫感染后免疫应答分析、疫苗效果评估或免疫调节药物开发等实验场景，为探索犬类免疫调控网络提供精准的检测工具。本产品仅限科学研究使用，需严格按照说明书操作以保证数据可靠性。
  • 别名：
    IL4Interleukin-4 ELISA Kit; IL-4 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BSF-1 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit
  • 缩写：
    IL4
  • Uniprot No.：
  • 种属：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    6.25 pg/mL-400 pg/mL
  • 灵敏度：
    1.56 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %94
    Range %87-97
    1:2Average %89
    Range %85-99
    1:4Average %98
    Range %92-105
    1:8Average %93
    Range %86-100
  • 回收率：
    The recovery of dog IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9590-105
    EDTA plasma (n=4)9285-96
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    4002.912 2.986 2.949 2.853
    2002.546 2.531 2.539 2.443
    1001.780 1.831 1.806 1.710
    501.127 1.207 1.167 1.071
    250.546 0.588 0.567 0.471
    12.50.259 0.276 0.268 0.172
    6.250.168 0.176 0.172 0.076
    00.095 0.096 0.096 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

产品评价

相关产品

IL4 antibodies

IL4 Proteins

靶点详情

  • 功能：
    Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
  • 基因功能参考文献：
    1. immunoreactivities of inflammatory cytokines, such as interleukin (IL)-2, its receptor (IL-2R), IL-4 and its receptor (IL-4R) in the cervical and lumbar spinal cord of young adult and aged beagle dogs, were compared. PMID: 23605681
    2. In dogs with leishmaniasis, there was high expression of genes encoding IL-4 in blood leucocytes. The predominance of IL-4 gene expression in the blood of asymptomatic dogs may favour parasite replication. PMID: 21511273
    3. The anti-inflammatory and anabolic effects of regulated expression of IL-4 in chondrocyte-scaffolds under in vitro inflammatory conditions, were investigated. PMID: 21991344
    4. This study focuses on the co-expression of interleukin-4 (IL-4) and insulin-like-growth factor-1 (IGF-1), which specifically target inflammation and cartilage repair, respectively. PMID: 20188584
    5. These findings suggest that polyunsaturated fatty acids are able to influence proliferation of peripheral blood mononuclear cells in healthy and atopic dogs but do not seem to influence cytokine transcription. PMID: 20187917
    6. IL-4 expressed in canine articular chondrocytes is biologically active and suppresses inflammatory mediators in vitro. PMID: 18789870

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    IL-4/IL-13 family
  • 数据库链接：

    KEGG: cfa:403785

    STRING: 9615.ENSCAFP00000001253

    UniGene: Cfa.39

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