Fish Acetylcholinesterase(AChE)ELISA Kit

文献引用 (8) 说明书下载

产品详情

产品描述：

CUSABIO鱼类乙酰胆碱酯酶（AChE）酶联免疫检测试剂盒（货号：CSB-E17001Fh），适用于定量分析鱼类血清、血浆及组织匀浆样本中的AChE水平，检测范围为2.5-40 ng/mL。乙酰胆碱酯酶是神经系统中关键的胆碱酯酶，参与乙酰胆碱的分解代谢，其活性变化常用于评估环境污染物（如有机磷农药）对水生生物神经系统的毒性效应。本试剂盒基于竞争法原理设计，通过特异性抗体与样本中AChE的结合反应实现精准定量，操作简便且灵敏度高，可在3小时内完成检测。该产品适用于科研领域的环境毒理学研究，例如水体污染物对鱼类神经功能的影响评估、生态毒性机制探究或实验室条件下鱼类模型的神经生化指标监测，为水产养殖安全、环境污染生物标志物筛选等研究提供可靠的数据支持。
别名：

acheAcetylcholinesterase ELISA kit; AChE ELISA kit; EC 3.1.1.7 ELISA kit
缩写：

AChE
Uniprot No.：

P04058
种属：

Fish
样本类型：

serum, plasma, tissue homogenates
检测范围：

2.5 ng/mL-40 ng/mL
灵敏度：

2.5 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of fish AChE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	89-103
1:2	Average %	92
	Range %	87-101
1:4	Average %	93
	Range %	87-100
1:8	Average %	89
	Range %	83-99

回收率：

The recovery of fish AChE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-93
EDTA plasma (n=4)	94	87-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. May be involved in cell-cell interactions.
亚细胞定位：

[Isoform H]: Cell membrane; Lipid-anchor, GPI-anchor. Cell junction, synapse.; [Isoform T]: Cell membrane; Peripheral membrane protein. Cell junction, synapse. Note=Attached to the membrane through disulfide linkage with the collagenic subunit, itself bound to the membrane.
蛋白家族：

Type-B carboxylesterase/lipase family
组织特异性：

Found in the synapses and to a lower extent in extrajunctional areas of muscle and nerve, and on erythrocyte membranes.