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Fish Catalase (CAT) ELISA kit

  • 中文名称:
    鱼过氧化氢酶(CAT)酶联免疫试剂盒
  • 货号:
    CSB-E15928Fh
  • 规格:
    96T/48T
  • 价格:
    ¥4200/¥3000
  • 其他:

产品详情

  • 产品描述:
    鱼过氧化氢酶(CAT)酶联免疫试剂盒(CSB-E15928Fh)为竞争法ELISA试剂盒,定量检测血清、血浆样本中的CAT含量。试剂盒检测范围为125 mU/mL-2000 mU/mL,该产品可应用于水产养殖研究中鱼类健康状态监测、水体污染对生物抗氧化系统的影响评估,以及环境毒理学中重金属、有机污染物等胁迫因子的效应机制研究,为鱼类生理生化研究提供可靠的实验工具本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 缩写:
  • Uniprot No.:
  • 种属:
    Fish
  • 样本类型:
    serum, plasma
  • 检测范围:
    125 mU/mL-2000 mU/mL
  • 灵敏度:
    62.5 mU/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Cancer
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of fish CAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 95  
    Range % 91-99  
    1:200 Average % 103  
    Range % 99-107  
    1:400 Average % 98  
    Range % 93-105  
    1:800 Average % 92  
    Range % 87-95  
  • 回收率:
    The recovery of fish CAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 99 94-104  
    EDTA plasma (n=4) 88 83-96  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mU/ml OD1 OD2 Average    
    2000 0.091 0.089 0.090    
    1000 0.242 0.257 0.250    
    500 0.553 0.587 0.570    
    250 0.913 0.980 0.947    
    125 1.647 1.563 1.605    
    0 2.381 2.319 2.350    
  • 数据处理:
  • 货期:
    3-5 working days

引用文献

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靶点详情

  • 最新研究进展:
    CAT是一种重要的抗氧化酶,在机体中起到清除自由基的作用,对细胞和组织的损伤具有保护作用。最新研究表明,CAT在代谢性疾病、肿瘤和神经系统等多个疾病的发生和发展中发挥着重要作用,通过抑制细胞凋亡、调节免疫反应等多种机制发挥保护作用,成为疾病治疗的潜在靶点。