Goat Heat Shock Protein 70(HSP-70) ELISA KIT
-
中文名称：
山羊热休克蛋白70(HSP-70)酶联免疫试剂盒
-
货号：
CSB-E13381G
-
规格：
96T/48T
-
价格：
￥3800/￥2500
-
其他：
产品详情
-
产品描述：
CUSABIO山羊热休克蛋白70（HSP-70）酶联免疫检测试剂盒，采用双抗体夹心ELISA原理实现定量分析，适用于血清、血浆及组织匀浆样本的精准检测。HSP-70作为一种重要的分子伴侣蛋白，在细胞应激、蛋白质折叠及免疫调节等生理病理过程中发挥关键作用，其表达水平变化常作为研究山羊应激反应、炎症或疾病状态的重要生物标志物。试剂盒检测范围覆盖23.44 ng/mL至1500 ng/mL的宽线性区间，可灵敏识别样本中HSP-70的浓度差异，操作流程标准化且重复性良好。在科研领域，该试剂盒可用于探究环境胁迫、病原感染或代谢异常等因素对山羊HSP-70表达的影响，亦适用于动物模型研究、生理机制解析及免疫调控相关实验，为畜牧科学、兽医学及基础生命科学研究提供可靠工具。试剂盒包含预包被捕获抗体、生物素化检测抗体及优化反应体系，支持高效完成样本检测与数据分析。
-
别名：
HSPA2 ELISA Kit; HSP70-3 ELISA Kit; Heat shock-related 70 kDa protein 2 ELISA Kit; Heat shock 70 kDa protein 3 ELISA Kit; HSP70.3 ELISA Kit
-
缩写：
-
Uniprot No.：
-
种属：
Capra hircus (Goat)
-
样本类型：
serum, plasma, tissue homogenates
-
检测范围：
23.44 ng/mL-1500 ng/mL
-
灵敏度：
5.86 ng/mL
-
反应时间：
1-5h
-
样本体积：
50-100ul
-
检测波长：
450 nm
-
研究领域：
Neuroscience
-
测定原理：
quantitative
-
测定方法：
Sandwich
-
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
-
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of goat HSP-70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 95 | |
| Range % | 90-99 | |
| 1:2 | Average % | 92 | |
| Range % | 85-99 | |
| 1:4 | Average % | 95 | |
| Range % | 92-103 | |
| 1:8 | Average % | 89 | |
| Range % | 82-98 | |
-
回收率：
| The recovery of goat HSP-70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 92 | 87-98 | |
| EDTA plasma (n=4) | 95 | 89-102 | |
| | | | | | | |
| | | | | | | |
-
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | ng/ml | OD1 | OD2 | Average | Corrected | | | 1500 | 2.308 | 2.463 | 2.386 | 2.234 | | | 750 | 1.606 | 1.666 | 1.636 | 1.484 | | | 375 | 1.078 | 1.064 | 1.071 | 0.919 | | | 187.5 | 0.732 | 0.721 | 0.727 | 0.575 | | | 93.75 | 0.428 | 0.456 | 0.442 | 0.290 | | | 46.88 | 0.313 | 0.330 | 0.322 | 0.170 | | | 23.44 | 0.219 | 0.235 | 0.227 | 0.075 | | | 0 | 0.151 | 0.153 | 0.152 | | |
|
-
数据处理：
-
货期：
3-5 working days
靶点详情
-
功能：
Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. It goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release. Plays a role in spermatogenesis. In association with SHCBP1L may participate in the maintenance of spindle integrity during meiosis in male germ cells.
-
亚细胞定位：
Cytoplasm, cytoskeleton, spindle.
-
蛋白家族：
Heat shock protein 70 family
Most popular with customers
