Goat Interleukin 4,IL-4 ELISA Kit

产品详情

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO山羊白介素4（IL-4）酶联免疫检测试剂盒（货号：CSB-E09808g），采用双抗体夹心法定量分析血清、血浆及组织匀浆中的IL-4浓度。IL-4是一种由Th2淋巴细胞分泌的关键细胞因子，在调节体液免疫、促进B细胞增殖分化及抗体类别转换中发挥重要作用，其水平变化可反映机体免疫状态与炎症反应进程。试剂盒检测灵敏度达15.6 pg/mL，线性范围为15.6-1000 pg/mL，适用于检测山羊不同生物样本中IL-4的动态表达。实验方案包含预包被特异性抗体的酶标板、标准品及检测试剂，通过标准曲线实现目标蛋白的精准定量。该试剂盒可应用于动物免疫学基础研究，如寄生虫感染模型中的Th2免疫应答评估、过敏性疾病机制探究或疫苗效价研究中的免疫指标分析，为科研人员解析山羊免疫调控网络提供可靠工具。本产品仅限科学研究使用，操作时需遵循标准实验室规范。
别名：

IL4Interleukin-4 ELISA Kit; IL-4 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BSF-1 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit
缩写：

IL4
Uniprot No.：

P79155
种属：

Capra hircus (Goat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of goat IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	102
	Range %	97-107
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	91-103

回收率：

The recovery of goat IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	96	90-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
亚细胞定位：

Secreted.
蛋白家族：

IL-4/IL-13 family
数据库链接：

KEGG: chx:100860814

UniGene: Chi.38713