Horse Gelsolin(GSN) ELISA kit

产品详情

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO马凝溶胶蛋白（Gelsoin, GSN）酶联免疫检测试剂盒（货号CSB-EL009965HO），采用竞争法原理实现定量分析，适用于马属动物血清、血浆及组织匀浆样本中GSN蛋白的检测。凝溶胶蛋白是一种钙离子依赖性肌动蛋白结合蛋白，广泛参与细胞骨架重塑、胞吐作用及炎症反应调控等生理过程，其表达水平变化与组织损伤修复、肿瘤转移等研究密切相关。本试剂盒检测灵敏度高，线性范围为12.5 ng/mL至800 ng/mL，通过预包被特异性抗体与样本/标准品中的GSN竞争结合标记物，结合显色反应实现精准定量。该产品可为马属动物细胞迁移机制、炎症相关疾病模型或肌肉组织再生等基础研究提供关键数据支持，适用于实验室条件下对生物样本中GSN蛋白表达水平的动态监测及比较分析，为探索其分子功能及调控机制提供可靠工具。
别名：

GSNGelsolin ELISA kit; Actin-depolymerizing factor ELISA kit; ADF ELISA kit; Brevin ELISA kit
缩写：

GSN
Uniprot No.：

Q28372
种属：

Equus caballus (Horse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

12.5 ng/mL-800 ng/mL
灵敏度：

6.25 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of horse GSN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	101
	Range %	94-105
1:2000	Average %	89
	Range %	82-94
1:4000	Average %	102
	Range %	98-106
1:8000	Average %	95
	Range %	91-99

回收率：

The recovery of horse GSN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-97
EDTA plasma (n=4)	91	87-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
基因功能参考文献：
1. Ca2+ binding by domain 2 plays a critical role in the activation and stabilization of gelsolin PMID: 19666512
亚细胞定位：

Cytoplasm, cytoskeleton.
蛋白家族：

Villin/gelsolin family
数据库链接：

KEGG: ecb:100034186

UniGene: Eca.2517