Human Acylamino-acid-releasing enzyme(APEH) ELISA kit

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产品详情

产品描述：

人酰基氨基酸释放酶(APEH)酶联免疫试剂盒（CSB-EL001899HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的APEH含量。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，科研人员可通过本产品研究 APEH 在细胞代谢调控、疾病发生机制中的作用，或用于药物开发中靶点活性评估。为体外蛋白水平研究提供高效工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

AARE ELISA Kit; ACPH ELISA Kit; ACPH_HUMAN ELISA Kit; Acyl peptide hydrolase ELISA Kit; Acyl-peptide hydrolase ELISA Kit; Acylamino acid releasing enzyme ELISA Kit; Acylamino-acid-releasing enzyme ELISA Kit; Acylaminoacyl peptidase ELISA Kit; Acylaminoacyl-peptidase ELISA Kit; APEH ELISA Kit; APH ELISA Kit; N acylaminoacyl peptide hydrolase ELISA Kit; OPH ELISA Kit; Oxidized protein hydrolase ELISA Kit
缩写：

APEH
Uniprot No.：

P13798
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.81 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human APEH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	82-87
1:2	Average %	98
	Range %	94-103
1:4	Average %	88
	Range %	84-92
1:8	Average %	108
	Range %	104-112

回收率：

The recovery of human APEH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	95-103
EDTA plasma (n=4)	86	86-89

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

This enzyme catalyzes the hydrolysis of the N-terminal peptide bond of an N-acetylated peptide to generate an N-acetylated amino acid and a peptide with a free N-terminus. It preferentially cleaves off Ac-Ala, Ac-Met and Ac-Ser.
基因功能参考文献：
1. Although APEH is primarily localized in the cytoplasm, a sub-fraction of this enzyme is sequestered at sites of nuclear damage following UVA irradiation or following oxidative stress. APEH interacts with the amino-terminal domain of XRCC1, and APEH facilitates both single-strand break repair and cell survival following exposure to H2O2. Thus, APEH as a novel proteolytic component of the DNA damage response. PMID: 28866241
2. APEH polymorphism has significant influence on valproic acid pharmacokinetics in Chinese population PMID: 27406852
3. Studies indicate that the cylindromatosis/turban tumor syndrome gene (CYLD) ranked highest, followed by acylaminoacyl-peptidase (APEH), dystroglycan (DAG1), macrophage-stimulating protein (MST1) and ubiquitin-specific peptidase 4 (USP4). PMID: 21931648
亚细胞定位：

Cytoplasm.
蛋白家族：

Peptidase S9C family
数据库链接：

HGNC: 586

OMIM: 102645

KEGG: hsa:327

STRING: 9606.ENSP00000296456

UniGene: Hs.517969