Human Angiotensin Ⅱ Receptor 2 antibody,AT2R-Ab ELISA Kit

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产品详情

产品描述：

CUSABIO试剂盒采用双抗体夹心法定量检测人血清或血浆样本中的血管紧张素Ⅱ受体2抗体（AT2R-Ab），适用于科研领域探索AT2R在生理病理过程中的作用机制。AT2R作为肾素-血管紧张素系统的重要成员，参与调节血管功能、细胞增殖及组织修复等生物学过程，其抗体水平变化可能与心血管疾病、纤维化疾病等研究相关。试剂盒检测范围为0.312-20 U/mL，仅需100μL样本即可完成检测，操作流程包含标准品稀释、样本孵育、洗涤及显色步骤，可在4.5小时内获得稳定结果。该产品采用高特异性配对抗体，确保对AT2R-Ab的精准识别，适用于基础研究中评估疾病模型动物的抗体动态变化、体外药物干预实验的效应分析，或筛选调控AT2R信号通路的潜在化合物。试剂盒提供预包被酶标板及完整检测组分，配套标准曲线可实现抗体浓度的准确定量，为心血管、肾脏等系统相关分子机制研究提供可靠工具。
别名：

angiotensin 2 receptor 2 antibody, AT2R-Ab
缩写：

AT2R Ab
种属：

Homo sapiens (Human)
样本类型：

serum, plasma
检测范围：

0.312 U/mL-20 U/mL
灵敏度：

0.078 U/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human AT2R-Ab in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	82-105
1:2	Average %	94
	Range %	84-100
1:4	Average %	98
	Range %	92-101
1:8	Average %	94
	Range %	82-105

回收率：

The recovery of human AT2R-Ab spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	83-105
EDTA plasma (n=4)	95	85-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.