Human Bone morphogenetic protein 3B(GDF10) ELISA kit
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中文名称：
人骨形态发生蛋白3B(GDF10)酶联免疫试剂盒
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货号：
CSB-EL009343HU
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规格：
96T/48T
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价格：
￥3600/￥2500
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其他：
产品详情
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产品描述：
CUSABIO人骨形态发生蛋白3B（GDF10）酶联免疫试剂盒（货号：CSB-EL009343HU），基于双抗体夹心法原理开发，适用于血清、血浆及组织匀浆样本中GDF10蛋白的定量检测。GDF10属于TGF-β超家族成员，在骨骼形态发生、细胞分化及组织修复中发挥重要调控作用，其表达水平与骨骼发育异常、纤维化疾病等病理过程密切相关。CUSABIO试剂盒采用高特异性配对抗体构建检测体系，可精确检测23.5-1500 pg/mL浓度范围内的目标蛋白，最低检测限达23.5 pg/mL，标准品线性相关系数R²＞0.99，具备良好的灵敏度和准确性。实验操作全程约需4.5小时，96孔板规格满足多样本批量检测需求，试剂盒组分包含标准品、预包被板及检测抗体等核心原料，建议保存于-20°C并避免反复冻融。该产品可广泛应用于骨骼发育机制研究、纤维化疾病模型分析、药物干预效果评价等科研领域，为探索GDF10在组织再生及病理调控中的分子机制提供可靠工具，实验结果仅用于科学研究。
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别名：
BIP ELISA Kit; BMP 3b ELISA Kit; BMP-3b ELISA Kit; BMP3B ELISA Kit; BMP3B_HUMAN ELISA Kit; Bone inducing protein ELISA Kit; Bone morphogenetic protein 3b [Precursor] ELISA Kit; Bone morphogenetic protein 3b ELISA Kit; Bone-inducing protein ELISA Kit; GDF 10 ELISA Kit; GDF-10 ELISA Kit; Gdf10 ELISA Kit; growth differentiation factor 10 ELISA Kit; Growth/differentiation factor 10 ELISA Kit
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缩写：
GDF10
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Uniprot No.：
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种属：
Homo sapiens (Human)
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样本类型：
serum, plasma, tissue homogenates
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检测范围：
23.5 pg/mL-1500 pg/mL
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灵敏度：
5.8 pg/mL
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反应时间：
1-5h
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样本体积：
50-100ul
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检测波长：
450 nm
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研究领域：
Signal Transduction
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测定原理：
quantitative
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测定方法：
Sandwich
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精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human GDF10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
107 |
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| Range % |
104-110 |
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| 1:2 |
Average % |
103 |
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| Range % |
101-105 |
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| 1:4 |
Average % |
88 |
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| Range % |
84-94 |
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| 1:8 |
Average % |
94 |
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| Range % |
91-97 |
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回收率：
| The recovery of human GDF10 piked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
91-103 |
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| EDTA plasma (n=4) |
87 |
85-97 |
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标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1500 |
1.956 |
2.121 |
2.039 |
1.906 |
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| 750 |
1.531 |
1.547 |
1.539 |
1.406 |
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| 375 |
1.101 |
1.112 |
1.107 |
0.974 |
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| 187.5 |
0.670 |
0.715 |
0.693 |
0.560 |
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| 94 |
0.424 |
0.457 |
0.441 |
0.308 |
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| 47 |
0.301 |
0.322 |
0.312 |
0.179 |
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| 23.5 |
0.221 |
0.235 |
0.228 |
0.095 |
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| 0 |
0.130 |
0.136 |
0.133 |
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数据处理：
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货期：
3-5 working days
靶点详情
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功能：
Growth factor involved in osteogenesis and adipogenesis. Plays an inhibitory role in the process of osteoblast differentiation via SMAD2/3 pathway. Plays an inhibitory role in the process of adipogenesis.
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基因功能参考文献：
- Study shows that GDF10 is down-regulated in patients with oral squamous cell carcinoma, and is an independent risk factor for overall survival. Its expression is regulated by TGFBR3 which shares the signaling inhibiting epithelial-mesenchymal transition. PMID: 25728212
- GDF10 is a stroke-induced signal for axonal sprouting and functional recovery. PMID: 26502261
- new hypoxia-inducible and SOX9-regulated genes, Gdf10 and Chm-I. In addition, Mig6 and InhbA were induced by hypoxia, predominantly via HIF-2alpha PMID: 18077449
- BMP3b and BMP6 genes were suppressed by DNA methylation and methylation of BMP3b is significantly frequent in Japanese malignant pleural mesotheliomas (MPMs), suggesting its pathogenic role and the ethnic difference in MPMs. PMID: 18949431
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亚细胞定位：
Secreted.
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蛋白家族：
TGF-beta family
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组织特异性：
Expressed in femur, brain, lung, skeletal muscle, pancreas and testis.
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数据库链接：
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