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  4. Human Bone morphogenetic protein 8B(BMP8B) ELISA kit

Human Bone morphogenetic protein 8B(BMP8B) ELISA kit

文献引用 (4) 说明书下载
  • 中文名称：
    人骨形态发生蛋白8B(BMP8B)酶联免疫试剂盒
  • 货号：
    CSB-EL002746HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    人骨形态发生蛋白8B（BMP8B）属于TGF-β超家族成员，在骨骼发育、胚胎生长及组织再生等生理过程中发挥重要调控作用，其表达水平的变化与多种疾病模型的研究密切相关。本试剂盒（货号CSB-EL002746HU）采用双抗体夹心法原理，可定量检测血清、血浆及组织匀浆样本中的BMP8B蛋白，线性检测范围为9.37-600 pg/mL，灵敏度达9.37 pg/mL，为科研工作者提供高特异性检测方案。试剂盒包含预包被捕获抗体的酶标板、生物素化检测抗体及标准品等核心组分，通过优化反应体系确保检测稳定性和重复性。该产品适用于体外研究BMP8B在骨代谢调控机制、生殖系统功能分析或特定病理模型（如骨发育异常、组织纤维化等）中的动态表达特征，为探索其分子作用机制提供可靠工具。实验操作流程简便，兼容常规实验室设备，支持多样本类型分析，尤其适用于需要高灵敏度检测的细胞或动物模型研究。
  • 别名：
    BMP-8 ELISA Kit; BMP-8B ELISA Kit; BMP8 ELISA Kit; Bmp8b ELISA Kit; BMP8B_HUMAN ELISA Kit; bone morphogenetic protein 8 (osteogenic protein 2) ELISA Kit; Bone morphogenetic protein 8 ELISA Kit; Bone morphogenetic protein 8B ELISA Kit; dJ118J21.1 (bone morphogenetic protein 8 (osteogenic protein 2)) ELISA Kit; OP-2 ELISA Kit; OP2 ELISA Kit; Osteogenic protein 2 ELISA Kit
  • 缩写：
    BMP8B
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    9.37 pg/mL-600 pg/mL
  • 灵敏度：
    2.34 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human BMP8B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:5 Average % 94  
    Range % 90-98  
    1:10 Average % 88  
    Range % 82-94  
    1:20 Average % 103  
    Range % 99-108  
    1:40 Average % 95  
    Range % 90-100  
  • 回收率：
    The recovery of human BMP8B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 92-100  
    EDTA plasma (n=4) 98 94-103  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    600 2.056 2.205 2.131 2.008  
    300 1.607 1.724 1.666 1.543  
    150 1.102 1.075 1.089 0.966  
    75 0.645 0.603 0.624 0.501  
    37.5 0.394 0.373 0.384 0.261  
    18.75 0.291 0.302 0.297 0.174  
    9.37 0.187 0.201 0.194 0.071  
    0 0.120 0.125 0.123    
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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BMP8B antibodies

BMP8B Proteins

靶点详情

  • 功能：
    Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis.
  • 基因功能参考文献：
    1. Acetylated H3K9 and H4K16 levels at BMP8B were increased in gastric cancer (GC) compared to nontumors (P < 0.05). However, reduced levels of acetylated H3K9 and H4K16 were associated with poorly differentiated GC (P < 0.05). PMID: 27748538
    2. Study found that BMP8B was downregulated in pancreatic cancer tissues and BMP8B mediates the survival of pancreatic cancer cells and regulates the progression of pancreatic cancer. PMID: 25176058
    3. Five novel SNPs within BMP8B gene.Genetic variants in BMP8B gene are associated with growth traits in Chinese native cattle. PMID: 24076131
    4. Epigenetic silencing of the bone morphogenetic protein 2 gene through methylation is associated with gastric carcinomas PMID: 16314833
    5. Reports on the mouse homolog of this gene. PMID: 9070944

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    TGF-beta family
  • 数据库链接：

    HGNC: 1075

    OMIM: 602284

    KEGG: hsa:656

    STRING: 9606.ENSP00000361915

    UniGene: Hs.734367

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