Human Calcitonin,CT ELISA Kit

产品详情

文献引用 (2) 说明书下载

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

人降钙素(CT)酶联免疫试剂盒（CSB-E05131h）检测血清、血浆、组织匀浆样本中的CALCA含量。CALCA 是重要靶点。它在机体生理活动里有关键作用。研究机制上，CALCA 参与多种信号通路，其表达异常和疾病发生发展关联紧密。科研聚焦于解析其作用途径，期望通过干预 CALCA 开发治疗疾病的新策略和药物。试剂盒检测范围为4.688 pg/mL-300 pg/mL，该产品适用于基础医学研究中CT蛋白表达水平的定量检测，如钙代谢调控机制探索、骨骼疾病动物模型评估或甲状腺功能相关病理研究，配套的血清/血浆前处理方案及组织匀浆制备指南可满足不同实验体系需求，为科研人员提供稳定可靠的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

CALCA ELISA kit; CALC1Calcitonin [Cleaved into: Calcitonin; Katacalcin ELISA kit; Calcitonin carboxyl-terminal peptide ELISA kit; CCP ELISA kit; PDN-21)] ELISA kit
缩写：

CALCA
Uniprot No.：

P01258
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

4.688 pg/mL-300 pg/mL
灵敏度：

1.172 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
Range %	81-95
1:2	Average %	95
Range %	92-100
1:4	Average %	94
Range %	90-110
1:8	Average %	94
Range %	89-105

回收率：

The recovery of human CT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	85-100
EDTA plasma (n=4)	92	88-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Calcitonin causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones.; Katacalcin is a potent plasma calcium-lowering peptide.
亚细胞定位：

Secreted.
蛋白家族：

Calcitonin family
数据库链接：

HGNC: 1437

OMIM: 114130

KEGG: hsa:796

STRING: 9606.ENSP00000331746

UniGene: Hs.37058