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Human Carboxyterminal propeptide of type Ⅰ procollagen,PⅠCP ELISA Kit

  • 中文名称:
    人Ⅰ型前胶原羧基端肽(PⅠCP)酶联免疫试剂盒
  • 货号:
    CSB-E08079h
  • 规格:
    96T/48T
  • 价格:
    ¥3600/¥2500
  • 其他:

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  • 产品描述:
    人Ⅰ型前胶原羧基端肽(PⅠCP)酶联免疫试剂盒(CSB-E08079h)为双抗夹心法ELISA试剂盒,定量检测血清、血浆、尿液样本中的PⅠCP含量。人Ⅰ型前胶原羧基端肽(PⅠCP)是反映骨代谢的重要指标,其水平变化与骨吸收和骨形成密切相关。研究显示,PⅠCP在骨质疏松、骨折愈合等疾病诊断和治疗监测中具有重要作用。试剂盒检测范围为62.5 pg/mL-4000 pg/mL,常用于骨转化研究、纤维化疾病模型评估及组织修复机制探索等科研领域。适用于骨代谢相关细胞实验、动物模型研究,以及肝纤维化、肺纤维化等疾病机制研究,尿液样本的兼容性为无创性研究提供了便利。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    Carboxyterminal propeptide of type 1 procollagen,P1CP,_x000D_ Carboxyterminal propeptide of type I procollagen,PICP
  • 缩写:
    PⅠCP
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, urine
  • 检测范围:
    62.5 pg/mL-4000 pg/mL
  • 灵敏度:
    15.6 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Others
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human PⅠCP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 95  
    Range % 99-101  
    1:200 Average % 91  
    Range % 85-97  
    1:400 Average % 87  
    Range % 82-94  
    1:800 Average % 95  
    Range % 90-100  
  • 回收率:
    The recovery of human PⅠCP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 87 81-93  
    EDTA plasma (n=4) 100 93-106  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    4000 2.356 2.397 2.377 2.184  
    2000 2.004 2.049 2.027 1.834  
    1000 1.633 1.674 1.654 1.461  
    500 1.221 1.265 1.243 1.050  
    250 0.869 0.855 0.862 0.669  
    125 0.578 0.598 0.588 0.395  
    62.5 0.367 0.398 0.383 0.190  
    0 0.196 0.190 0.193    
  • 数据处理:
  • 货期:
    3-5 working days

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