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中文名称：人几丁质酶3样蛋白2(CHI3L2)酶联免疫试剂盒
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货号：CSB-EL005347HU
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规格：96T/48T
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价格：￥3600/￥2500
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其他：
产品详情
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产品描述：本试剂盒（货号：CSB-EL005347HU）采用双抗体夹心法对人几丁质酶3样蛋白2（CHI3L2）进行定量检测，适用于血清、血浆及组织匀浆样本的科研分析。CHI3L2作为几丁质酶家族成员，在炎症反应、细胞外基质重塑及组织修复中发挥重要作用，其表达水平与多种病理过程相关。试剂盒灵敏度达0.78 ng/ml，检测范围为0.78-50 ng/ml，通过预包被特异性抗体实现高精度捕获靶蛋白，结合酶标二抗显色系统完成定量分析，操作流程兼容常规实验室条件。该产品可为炎症性疾病机制研究、纤维化模型评估或肿瘤微环境分析提供关键生物标志物检测支持，特别适用于基础医学、免疫学及分子病理学领域的体外样本检测，帮助研究者探索CHI3L2在疾病发生发展中的调控作用。
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别名：CH3L2_HUMAN ELISA Kit; CHI3L2 ELISA Kit; Chitinase 3 like 2 ELISA Kit; Chitinase-3-like protein 2 ELISA Kit; Chondrocyte protein 39 ELISA Kit; OTTHUMP00000014028 ELISA Kit; YKL 39 ELISA Kit; YKL-39 ELISA Kit; YKL39 ELISA Kit
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缩写：
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Uniprot No.：
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种属：Homo sapiens (Human)
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样本类型：serum, plasma, tissue homogenates
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检测范围：0.78 ng/ml-50 ng/ml
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灵敏度：0.19 ng/ml
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Stem Cells
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测定原理：quantitative
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测定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
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线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human CHI3L2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
87
Range %
81-91
1:2
Average %
101
Range %
95-105
1:4
Average %
102
Range %
98-109
1:8
Average %
96
Range %
91-98
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回收率：
The recovery of human CHI3L2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
94
89-96
EDTA plasma (n=4)
96
91-98
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标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml
OD1
OD2
Average
Corrected
50
2.701
2.688
2.695
2.582
25
2.344
2.192
2.268
2.155
12.5
1.704
1.729
1.717
1.604
6.25
1.151
1.137
1.144
1.031
3.125
0.678
0.699
0.689
0.576
1.56
0.388
0.366
0.377
0.264
0.78
0.238
0.241
0.240
0.127
0
0.115
0.111
0.113
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数据处理：
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货期：3-5 working days
引用文献
- Chitinase 3-like proteins as diagnostic and prognostic biomarkers of multiple sclerosis Hinsinger G et al,Mult Scler,2015
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靶点详情
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功能：Lectin that binds chitooligosaccharides and other glycans with high affinity, but not heparin. Has no chitinase activity.
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基因功能参考文献：
- This study shown that CHI3L2 is regulated differently in healthy and late onset Alzheimer"s disease brain depending on the sex. PMID: 27653898
- Structural analysis demonstrates that YKL-39 interacts with chitooligosaccharides through hydrogen bonds and hydrophobic interactions. Thermodynamic analysis indicates that binding of chitooligosaccharide to YKL-39 is mainly driven by enthalpy. PMID: 25477513
- These data suggest that hYKL-39 is a novel growth and differentiation factor involved in cartilage homeostasis. PMID: 23291184
- YKL-39 possesses a chitinase-like fold, but lacks key active-site residues required for catalysis. PMID: 22742450
- ERK1/2 phosphorylation by CHI3L2 inhibits cell mitogenesis and proliferation. PMID: 22211103
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亚细胞定位：Secreted.
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蛋白家族：Glycosyl hydrolase 18 family
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组织特异性：Highest expression in chondrocytes, followed by synoviocytes, lung and heart. Not detected in spleen, pancreas, and liver. May also be expressed in developing brain and placenta.
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数据库链接：
