Human Collagen Type Ⅱ,Col Ⅱ ELISA Kit

产品详情

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO人Ⅱ型胶原（Col Ⅱ）酶联免疫检测试剂盒（货号：CSB-E08087h），采用双抗体夹心法定量检测血清、血浆或组织匀浆样本中的Col Ⅱ含量。Ⅱ型胶原是软骨组织细胞外基质的主要成分，在维持软骨结构完整性和力学特性中起关键作用，其表达水平变化与骨关节炎、软骨损伤修复及关节退行性疾病的研究密切相关。本试剂盒检测范围为31.25-2000 pg/mL，可精准覆盖生物样本中Col Ⅱ的常见浓度范围，适用于体外科研实验中定量分析Col Ⅱ的表达动态，例如研究软骨组织工程材料的生物相容性、关节炎模型中基质代谢变化、软骨细胞分化调控机制等。试剂盒包含预包被特异性抗体的酶标板、标准品及检测所需试剂，操作流程标准化，可在4小时内完成检测，为软骨相关疾病机制探索、药物干预效果评估等基础研究提供可靠工具。
别名：

Collagen Type 2,Col 2,_x000D_ Collagen Type II,Col II
缩写：

Col Ⅱ
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.8 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human Col Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	86
	Range %	80-90
1:200	Average %	93
	Range %	88-98
1:400	Average %	94
	Range %	90-100
1:800	Average %	96
	Range %	90-102

回收率：

The recovery of human Col Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-98
EDTA plasma (n=4)	95	90-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.