Human Collagen Type Ⅳ(COL Ⅳ) ELISA Kit

产品详情

文献引用 (1) 说明书下载

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

人Ⅳ型胶原(Col Ⅳ)酶联免疫试剂盒（CSB-E17116h）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、尿液样本中的Col Ⅳ含量。Col Ⅳ（IV型胶原蛋白）是细胞基底膜的主要结构成分，参与组织完整性维持和正常功能。其研究机制涉及细胞外基质组成、细胞黏附、迁移、生存、扩张和分化。在疾病如肿瘤、肝纤维化等中，Col Ⅳ的表达和功能异常，成为治疗研究的重要靶点。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，本产品适用于体外研究基底膜相关疾病机制、评估药物对细胞外基质代谢的影响，或通过体液样本分析Col Ⅳ作为生物标志物的潜在价值，为组织修复、血管生成及纤维化研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Collagen Type 4(COL 4),_x000D_ Collagen Type IV(COL IV)
缩写：

Col Ⅳ
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, cell culture supernates, urine
检测范围：

62.5 pg/mL-4000 pg/mL
灵敏度：

15.6 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human COL Ⅳ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	86
	Range %	80-104
1:400	Average %	97
	Range %	91-105
1:800	Average %	100
	Range %	92-113
1:1600	Average %	93
	Range %	86-98

回收率：

The recovery of human COL Ⅳ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	89-102
EDTA plasma (n=4)	93	85-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.