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中文名称：人Ⅰ型胶原(Col Ⅰ)酶联免疫试剂盒
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货号：CSB-E08082h
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规格：96T/48T
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价格：￥3600/￥2500
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其他：
产品详情
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产品描述：人Ⅰ型胶原(Col Ⅰ)酶联免疫试剂盒（CSB-E08082h）为双抗夹心法ELISA试剂盒，定量检测血清、尿液、组织培养上清液、组织匀浆样本中的Col Ⅰ含量。试剂盒检测范围为1.56 ng/mL-100 ng/mL，适用于纤维化疾病模型研究、骨代谢机制探索以及再生医学中胶原沉积的动态分析。该产品兼容多种实验场景，包括体外细胞培养体系中胶原分泌的定量评估、动物模型组织修复过程的生物标志物追踪，以及临床前研究中Col Ⅰ代谢相关病理生理机制的解析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
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别名：Collagen Type 1,Col 1,_x000D_Collagen Type I,Col I
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缩写：Col Ⅰ
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种属：Homo sapiens (Human)
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样本类型：serum, urine, cell culture supernates, tissue homogenates
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检测范围：1.56 ng/mL-100 ng/mL
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灵敏度：0.39 ng/mL
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Signal Transduction
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测定原理：quantitative
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测定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human Col Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 87 Range % 80-92 1:2 Average % 100 Range % 91-110 1:4 Average % 105 Range % 92-110 1:8 Average % 93 Range % 86-100 -
回收率：
The recovery of human Col Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 94 89-99 -
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
ng/ml OD1 OD2 Average Corrected 100 2.921 2.985 2.953 2.767 50 2.356 2.378 2.367 2.181 25 1.826 1.875 1.851 1.665 12.5 1.221 1.256 1.239 1.053 6.25 0.796 0.800 0.798 0.612 3.12 0.512 0.546 0.529 0.343 1.56 0.302 0.326 0.314 0.128 0 0.188 0.184 0.186 -
数据处理：
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货期：3-5 working days
引用文献
- Tuberostemonine may alleviates proliferation of lung fibroblasts caused by pulmonary fibrosis A Tang, Y Liu, Q Ding, G Huang, Z Sha,International Journal of Immunopathology and Pharmacology,2024
- The Role of Collagen I, Magnesium and Phosphorus in Iraqi Meningioma Patients RZ Jasim,/,2024
- Skin antiaging effects of a multiple mechanisms hyaluronan complex F Chen,Skin Research and Technology,2023
- Secretome of human umbilical cord mesenchymal stem cell maintains skin homeostasis by regulating multiple skin physiological function X Wang,Cell and Tissue Research,2022
- Graphene Oxide Loaded on TiO2-Nanotube-Modified Ti Regulates the Behavior of Human Gingival Fibroblasts X Cao,International journal of molecular sciences,2022
- Contribution of Adiponectin/Carnitine Palmityl Transferase 1A-Mediated Fatty Acid Metabolism during the Development of Idiopathic Pulmonary Fibrosis W Wu,Oxidative Medicine and Cellular Longevity,2022
- Fermented Dendrobium officinale polysaccharides protect UVA‐induced photoaging of human skin fibroblasts Y Zhang,Food Science & Nutrition,2022
- Responses of human gingival fibroblasts to superhydrophilic hydrogenated titanium dioxide nanotubes C Wang,Colloids and Surfaces B: Biointerfaces,2020
- Biocompatibility and antibacterial properties of pure titanium surfaces coated with yttrium-doped hydroxyapatite Zhang K, et al,Journal of The Mechanical Behavior of Biomedical Materials,2019
- Chemical Fingerprint of Zn-Hydroxyapatite in the Early Stages of Osteogenic Differentiation Procopio A, et al,Pulmonary Pharmacology & Therapeutics,2019
产品评价
平均分:
样品类型：血清 ,细胞培养上清液
样品信息：人
稀释比：其他 1:10
产品评价： human collagen type1 COL1 ELISA试剂盒检测人血清/血浆，正常、模型和给药组样本数据很稳定，复孔差异较小。血清/血浆结果相近，COL1灵敏度比COL3高，试剂盒稳定方便。
By D老师
样品类型：细胞培养上清液
样品信息：人
稀释比：其他 1:5
产品评价： 使用CSB-E08082h试剂盒检测细胞中collagen 1含量，标曲趋势正确，数据完好。
By 张老师
相关产品
靶点详情
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最新研究进展：Collagen Ⅰ（Ⅰ型胶原）是一种主要存在于结缔组织中的蛋白质，最新研究表明它在疾病发生和治疗中也起着重要作用。例如，肝纤维化和骨质疏松等疾病都与胶原Ⅰ的异常表达有关。此外，一些药物和生物制剂已经用于调节胶原Ⅰ的合成和代谢，以治疗相关疾病。
