Human Corticosterone,CORT ELISA Kit

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产品详情

产品描述：

人皮质酮/肾上腺酮(CORT)酶联免疫试剂盒（CSB-E07968h）为竞争法ELISA试剂盒，定量检测血清、血浆样本中的CORT含量。CORT（皮质酮受体）在生理上参与应激反应等重要活动。其研究机制主要聚焦于它在神经内分泌系统中的信号传导途径，探究其与糖皮质激素的结合作用，以及对下游基因表达调控的影响，有助于理解应激相关疾病的发病机理。试剂盒检测范围为0.5 ng/mL-20 ng/mL，适用于神经内分泌研究、代谢综合征模型构建、慢性压力相关动物实验等科研场景，为探究HPA轴（下丘脑 - 垂体 - 肾上腺轴）功能、糖皮质激素代谢通路及药物干预效果提供高效工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

N/A
缩写：

CORT
种属：

Homo sapiens (Human)
样本类型：

serum, plasma
检测范围：

0.5 ng/mL-20 ng/mL
灵敏度：

0.5 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CORT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	103
	Range %	99-108
1:2	Average %	92
	Range %	88-96
1:4	Average %	96
	Range %	98-100
1:8	Average %	90
	Range %	85-94

回收率：

The recovery of human CORT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-93
EDTA plasma (n=4)	94	90-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.