Human EGF, latrophilin and seven transmembrane domain-containing protein 1(ELTD1) ELISA kit

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产品详情

产品描述：

人EGF、嗜乳蛋白和7跨膜结构域蛋白1(ELTD1)酶联免疫试剂盒（CSB-EL007630HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的ELTD1含量。试剂盒检测范围为25 pg/mL-1600 pg/mL，适用于基础医学研究中相关蛋白的表达水平检测，例如细胞功能调控机制探索、疾病动物模型分析或生物标志物筛选等实验场景，为肿瘤生物学、再生医学及代谢疾病研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

ADGRL4; ELTD1; ETL; UNQ202/PRO228; Adhesion G protein-coupled receptor L4; EGF, latrophilin and seven transmembrane domain-containing protein 1; EGF-TM7-latrophilin-related protein; ETL protein
缩写：

ELTD1
Uniprot No.：

Q9HBW9
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

25 pg/mL-1600 pg/mL
灵敏度：

6.25 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human ELTD1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	89-98
1:2	Average %	97
	Range %	91-103
1:4	Average %	92
	Range %	87-98
1:8	Average %	88
	Range %	85-92

回收率：

The recovery of human ELTD1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	98-104
EDTA plasma (n=4)	87	82-91

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Endothelial orphan receptor that acts as a key regulator of angiogenesis.
基因功能参考文献：
1. High ELTD1 expression is associated with glioma. PMID: 27416955
2. miR-139-5p suppresses glioblastoma multiforme cell proliferation by targeting ELTD1 and regulating cell cycle. PMID: 26449464
3. Results provide insight into the regulation of tumor angiogenesis and highlight ELTD1 as key player in blood vessel formation. PMID: 23871637
4. Two of these (D1S0818i-ELTD1 and D6S0035i-MAPK14) remain associated following application of Bonferroni's correction and multivariate analysis. PMID: 23474535
5. Results of this study strongly suggests that associative analysis was able to accurately identify ELTD1 as a putative glioma-associated biomarker PMID: 23096411
6. Genes such as ELTD1 on chromosome 1, in addition to genes on chromosomes 4 (eg, GABRA2) and 6 (eg, CNR1), may be associated with the genetic risk for cannabis use disorders. PMID: 18519829
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亚细胞定位：

Cell membrane; Multi-pass membrane protein.
蛋白家族：

G-protein coupled receptor 2 family, Adhesion G-protein coupled receptor (ADGR) subfamily
组织特异性：

Detected in the majority of epithelial cells in tumor and normal tissues. Expressed also in human umbilical vein endothelial cells.
数据库链接：

HGNC: 20822

OMIM: 616419

KEGG: hsa:64123

STRING: 9606.ENSP00000359778

UniGene: Hs.132314