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  4. Human Endosialin(CD248) ELISA kit

Human Endosialin(CD248) ELISA kit

文献引用 (1) 说明书下载
  • 中文名称：
    人内皮唾酸蛋白(CD248)酶联免疫试剂盒
  • 货号：
    CSB-EL004905HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO人内皮唾酸蛋白(CD248)酶联免疫检测试剂盒（货号：CSB-EL004905HU），采用双抗体夹心法实现定量分析，适用于血清、血浆、组织匀浆及细胞裂解液样本中CD248蛋白的精准检测。CD248是一种表达于内皮细胞和肿瘤相关成纤维细胞表面的跨膜糖蛋白，参与血管生成、组织修复及病理微环境调控，在肿瘤生长、纤维化疾病等研究领域具有重要科研价值。该试剂盒检测灵敏度达0.312 ng/mL，线性范围为0.312-20 ng/mL，可稳定检测样本中痕量至中高浓度的CD248蛋白。其高特异性抗体组合能有效避免交叉反应，确保实验数据的可靠性。CUSABIO研究CD248在实体瘤血管新生机制、炎症微环境动态变化、纤维化疾病进展等方向提供标准化工具，适用于细胞生物学实验、疾病模型动物研究及生物标志物探索等科研场景，助力靶向治疗基础研究与病理机制解析。
  • 别名：
    CD164 sialomucin like 1 ELISA Kit; CD164L1 ELISA Kit; CD248 ELISA Kit; CD248 antigen endosialin ELISA Kit; CD248 molecule endosialin ELISA Kit; CD248_HUMAN ELISA Kit; Endosialin ELISA Kit; TEM 1 ELISA Kit; TEM1 ELISA Kit; Tumor endothelial marker 1 ELISA Kit
  • 缩写：
    CD248
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CD248 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %86
    Range %80-90
    1:2Average %97
    Range %90-105
    1:4Average %96
    Range %91-100
    1:8Average %94
    Range %89-100
  • 回收率：
    The recovery of human CD248 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9080-98
    EDTA plasma (n=4)9691-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    201.909 1.852 1.881 1.741
    101.696 1.706 1.701 1.561
    51.444 1.411 1.428 1.288
    2.50.952 0.993 0.973 0.833
    1.250.583 0.549 0.566 0.426
    0.6250.382 0.395 0.389 0.249
    0.3120.218 0.201 0.210 0.070
    00.142 0.137 0.140 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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CD248 antibodies

CD248 Proteins

靶点详情

  • 功能：
    May play a role in tumor angiogenesis.
  • 基因功能参考文献：
    1. These findings identify novel protein interactions involving CLEC14A, CD93 and CD248 with MMRN2 as targetable components of vessel formation PMID: 28671670
    2. Data show that podoplanin (PDPN), CD106 (VCAM-1) and CD248 protein were increased in diseased compared to healthy tendon cells. PMID: 28122639
    3. the data demonstrate a critical role for endosialin-expressing primary tumor pericytes in mediating metastatic dissemination and identify endosialin as a promising therapeutic target in breast cancer PMID: 27635044
    4. Endosialin is a potential regulator of phenotypic remodeling of vascular smooth muscle cells contributing to atherosclerosis. PMID: 28126825
    5. Tumour cell endosialin expression was seen in 89% of undifferentiated pleomorphic sarcomas, 77% of adult fibrosarcomas/spindle cell sarcomas, 62% of synovial sarcomas, 51% of leiomyosarcomas and 31% of rhabdomyosarcomas. PMID: 27434038
    6. CD248 overexpression is possibly involved in the pathogenesis of IPF and it has potential as a disease severity marker. PMID: 27080864
    7. TEM1 expression in cancer-associated fibroblasts is correlated with a poor prognosis in patients with gastric cancer PMID: 26336878
    8. MORAb-004 reduced CD248 on pericytes, impaired tumor microvasculature maturation and ultimately suppressed tumor development. PMID: 26327620
    9. The authors show that endosialin, a C-type lectin, expressed in the liver exclusively by HSC and portal fibroblasts, is upregulated in liver fibrosis in mouse and man. PMID: 25680861
    10. The genes CD248, Ephb1 and P2RY2 were detected as the top overexpressed in GC biopsies. PMID: 24716914
    11. new monoclonal antibodies described in this paper are domain-specific and represent important reagents for the study of functional contributions of extracellular endosialin domains PMID: 22824847
    12. [review] CD248 is a C-type lectin-like domain-containing cell surface glycoprotein that is expressed by stromal cells of proliferating tissues during embryogenesis and postnatally in tumors and inflammatory lesions. PMID: 22206249
    13. CD248 defines a subset of stromal cells, including but not limited to some myofibroblasts, linked to albuminuria and tubulointerstitial damage during tissue remodeling in CKD. PMID: 21490589
    14. endosialin can be detected in advanced sarcoma PMID: 21537839
    15. Endosialin expression may be involved in the progression of rectal cancers. PMID: 21362178
    16. Results provide evidence for a TEM-1-dependent signal pathway that controls proliferation of pericytes. PMID: 20484976
    17. TEM1/endosialin was induced in the vasculature of high-grade brain tumors where its expression was inversely correlated with patient age. PMID: 19948061
    18. Endosialin colocalized with thrombomodulin, suggesting the proteins may have complementary functions in tumor progression. PMID: 15624764
    19. Endosialin is a marker of stromal fibroblasts and is not selectively expressed on tumor endothelium. PMID: 15862292
    20. results pinpoint to a molecular mechanism by which expression of endosialin/TEM1 in the tumor stroma and endothelium may support tumor progression and invasion PMID: 17986615
    21. experiments unambiguously demonstrate that endosialin is expressed by tumor-associated myofibroblasts & mural cells & not by endothelial cells; results validate endosialin as marker of tumor-associated myofibroblasts & tumor vessel-associated mural cells PMID: 18187565
    22. endosialin is not expressed in normal human adult brain but is strongly upregulated in the angiogenic vasculature of all high-grade glioma specimens examined PMID: 18192970
    23. identified extracellular endosialin ligands and identified Mac-2 BP/90K as a specific interaction partner PMID: 18490383
    24. expression of TEM1 or endosialin (CD248) and other TEM has been discovered in a population of vascular endothelial growth factor receptor 2+/CD31+/CD45-/VE-cadherin+ EPC derived from human CD133+/CD34+ cells PMID: 18723498
    25. endosialin is strongly expressed by pericytes during periods of active angiogenesis during embryonic and tumor development PMID: 18761022
    26. These findings elucidate important aspects of endosialin gene regulation PMID: 18813310

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  • 亚细胞定位：
    Membrane; Single-pass type I membrane protein.
  • 组织特异性：
    Expressed in tumor endothelial cells but absent or barely detectable in normal endothelial cells. Expressed in metastatic lesions of the liver and during angiogenesis of corpus luteum formation and wound healing. Expressed in vascular endothelial cells of
  • 数据库链接：

    HGNC: 18219

    OMIM: 606064

    KEGG: hsa:57124

    STRING: 9606.ENSP00000308117

    UniGene: Hs.195727

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