Human Epigen(EPGN) ELISA kit

文献引用 (1)

产品详情

产品描述：

CUSABIO人Epigen（EPGN）酶联免疫检测试剂盒（货号CSB-EL007719HU），采用双抗体夹心法定量检测血清、血浆或组织匀浆样本中的EPGN蛋白。Epigen属于表皮生长因子（EGF）家族成员，在细胞增殖、迁移、组织修复及肿瘤发生发展中发挥调控作用，其表达水平与炎症反应、肿瘤微环境调控等研究密切相关。试剂盒检测范围为1.56 ng/mL至100 ng/mL，实验操作全程约需4小时，包含预包被特异性抗体的96孔板及标准品、检测抗体、显色底物等必要组分，可通过酶标仪在450 nm波长下读取吸光度值并生成标准曲线进行定量分析。本试剂盒适用于体外研究EPGN在肿瘤生物学、细胞信号通路或炎症相关分子机制中的表达特征，支持多种样本类型检测，具有高灵敏度和特异性，可满足基础科研中对EPGN蛋白表达动态变化的精确分析需求。
别名：

ALGV3072 ELISA Kit; EPG ELISA Kit; EPGN ELISA Kit; EPGN_HUMAN ELISA Kit; Epigen ELISA Kit; Epigen precursor ELISA Kit; Epithelial mitogen ELISA Kit; PRO9904 ELISA Kit
缩写：

EPGN
Uniprot No.：

Q6UW88
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

1.56 ng/mL-100 ng/mL
灵敏度：

0.39 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human EPGN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	103
	Range %	96-107
1:2	Average %	102
	Range %	97-105
1:4	Average %	92
	Range %	85-96
1:8	Average %	97
	Range %	92-101

回收率：

The recovery of human EPGN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-103
EDTA plasma (n=4)	105	98-109

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Promotes the growth of epithelial cells. May stimulate the phosphorylation of EGFR and mitogen-activated protein kinases.
基因功能参考文献：
1. Study shows how the EGFR ligands epiregulin (EREG) and epigen (EPGN) stabilize different dimeric conformations of the EGFR extracellular region. Results reveal how responses to different EGFR ligands are defined by receptor dimerization strength and signaling dynamics. These findings have broad implications for understanding receptor tyrosine kinase (RTK) signaling specificity. PMID: 28988771
2. cloning of a related gene in mouse PMID: 112783
3. EPG is an EGF-like growth factor and a low affinity ligand PMID: 15611079
亚细胞定位：

[Isoform 1]: Membrane; Single-pass type I membrane protein.; [Isoform 2]: Membrane; Single-pass type I membrane protein.; [Isoform 3]: Secreted.; [Isoform 4]: Secreted.; [Isoform 5]: Secreted.; [Isoform 6]: Secreted.
数据库链接：

HGNC: 17470

KEGG: hsa:255324

UniGene: Hs.401237