Human Epinephrine/Adrenaline,EPI ELISA Kit

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO人肾上腺素（Epinephrine, EPI）酶联免疫试剂盒（货号：CSB-E08677h），适用于定量检测血清、血浆及组织匀浆样本中EPI的含量。肾上腺素作为肾上腺髓质分泌的关键激素，在应激反应、心血管功能调节及代谢活动中发挥重要作用，其水平变化与神经内分泌调控、压力生理及代谢性疾病等研究密切相关。CUSABIO试剂盒采用竞争法检测原理，通过样本中EPI与标记物竞争结合特异性抗体的特性实现定量分析，检测范围覆盖2-400 pg/mL的宽泛线性区间，可精准捕捉低浓度样本的细微变化。该试剂盒操作便捷，兼容血清、血浆样本直接检测及组织匀浆样本分析，无需复杂前处理，适用于神经生物学、内分泌学及药理学等领域的基础研究，例如应激状态下激素分泌机制探索、心血管疾病模型中的激素动态监测，或药物干预对肾上腺功能影响的评价等科研场景，为相关机制研究提供可靠工具。
缩写：

EPI
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

2 pg/mL-400 pg/mL
灵敏度：

1 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human EPI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-98
1:2	Average %	95
	Range %	90-99
1:4	Average %	90
	Range %	85-96
1:8	Average %	90
	Range %	84-98

回收率：

The recovery of human EPI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	80-94
EDTA plasma (n=4)	98	92-107