Human Fibroblast growth factor 17(FGF17) ELISA kit
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中文名称：人成纤维细胞生长因子17(FGF17)酶联免疫试剂盒
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货号：CSB-EL008622HU
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规格：96T/48T
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价格：￥3600/￥2500
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其他：
产品详情
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产品描述：CUSABIO人成纤维细胞生长因子17（FGF17）酶联免疫吸附测定试剂盒（货号：CSB-EL008622HU），采用双抗体夹心法原理实现定量检测，适用于科研领域中对血清、血浆、组织匀浆及细胞培养上清等样本中FGF17蛋白含量的分析。FGF17属于成纤维细胞生长因子家族成员，在胚胎发育、组织修复及代谢调控中发挥重要作用，其异常表达可能与神经系统疾病、肿瘤发生等病理过程相关。该试剂盒检测灵敏度为3.12 pg/ml，线性检测范围覆盖3.12-200 pg/ml，实验流程约需3小时完成，提供预包被96孔板及标准品、检测抗体等核心组分，需配合酶标仪在450 nm波长下读取吸光度值。研究者可将其应用于发育生物学、疾病机制研究或药物干预实验中FGF17表达水平的动态监测，为探索其生理功能及病理意义提供可靠的工具支持。本试剂盒需在2-8°C条件下保存，开封后建议按说明书分装冻存以保持稳定性。
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别名：FGF 13 ELISA Kit; FGF 17 ELISA Kit; FGF-17 ELISA Kit; FGF13 ELISA Kit; Fgf17 ELISA Kit; FGF17_HUMAN ELISA Kit; Fibroblast growth factor 17 ELISA Kit; HH20 ELISA Kit
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缩写：
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Uniprot No.：
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种属：Homo sapiens (Human)
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样本类型：serum, plasma, tissue homogenates, cell culture supernates.
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检测范围：3.12 pg/ml-200 pg/ml.
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灵敏度：0.78 pg/ml.
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Signal Transduction
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测定原理：quantitative
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测定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
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线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human FGF17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
90
Range %
85-96
1:2
Average %
95
Range %
88-101
1:4
Average %
100
Range %
93-105
1:8
Average %
93
Range %
86-101
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回收率：
The recovery of human FGF17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
95
86-100
EDTA plasma (n=4)
97
92-103
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标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml
OD1
OD2
Average
Corrected
200
2.264
2.234
2.249
2.078
100
1.582
1.667
1.625
1.454
50
0.986
1.028
1.007
0.836
25
0.660
0.629
0.645
0.474
12.5
0.453
0.431
0.442
0.271
6.25
0.338
0.343
0.341
0.170
3.12
0.251
0.244
0.247
0.077
0
0.166
0.175
0.171
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数据处理：
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货期：3-5 working days
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靶点详情
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功能：Plays an important role in the regulation of embryonic development and as signaling molecule in the induction and patterning of the embryonic brain. Required for normal brain development.
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基因功能参考文献：
- FGF17 and IL17RD prpopsed as the two top candidates in the entire proteome on the basis of a statistical test of their protein-protein interaction patterns to proteins known to be altered in congenital hypogonadotropic hypogonadism. PMID: 23643382
- FGF17 expression is increased 2-fold in benign prostatic hyperplasia and may contribute to the increased epithelial proliferation seen in this disease. PMID: 15129425
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相关疾病：Hypogonadotropic hypogonadism 20 with or without anosmia (HH20)
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亚细胞定位：Secreted.
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蛋白家族：Heparin-binding growth factors family
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组织特异性：Preferentially expressed in the embryonic brain.
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数据库链接：
