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Human Hepatitis E virus antibody(IgG)ELISA Kit

  • 中文名称:
    人戊型肝炎病毒抗体(IgG)酶联免疫试剂盒
  • 货号:
    CSB-E04811h
  • 规格:
    96T/48T
  • 价格:
    ¥3200/¥2500
  • 其他:

产品详情

  • 产品描述:
    CUSABIO人戊型肝炎病毒抗体(IgG)酶联免疫检测试剂盒(货号:CSB-E04811h),专为科研领域设计,用于定性检测血清或血浆样本中的HEV-IgG抗体。戊型肝炎病毒(HEV)主要通过粪口途径传播,感染后引发的IgG抗体通常在急性期后持续存在,是评估既往感染的重要指标。CUSABIO试剂盒采用间接法原理,通过抗原包被微孔板捕获样本中的特异性抗体,结合酶标二抗显色反应实现定性分析,适用于判断样本中抗体存在与否。检测样本类型涵盖血清与血浆,具体检测范围可根据实验需求进一步咨询获取。该产品可应用于HEV感染的流行病学调查研究、疫苗接种后抗体应答评估、群体免疫水平监测等科研场景,也可用于动物模型或体外实验中的抗体动态变化分析,为病毒传播规律研究及免疫机制探索提供技术支持。
  • 缩写:
    HEV Ab (IgG)
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma
  • 检测范围:
    Request Information
  • 灵敏度:
    Request Information
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Microbiology
  • 测定原理:
    qualitative
  • 测定方法:
    Indirect
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<15%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<15%

    Three samples of known concentration were tested in twenty assays to assess.

  • 标准曲线:

    Test parameter

    specification

    test result

    Positive control

    >0.30

    0.799

    Negative control

    <0.08

    0.046

    Positive rate

    10,Positive

    100%

    Negative rate

    10,Negative

    100%

  • 本试剂盒所含材料:
    • A 96-well Assay plate --The 96-well plate has been pre-coated with HEV antigen
    • Negative Control (1 x 0.4ml) --Eliminate false positive
    • Positive Control (1 x 0.4ml) --Used to evaluate the validity, stability, and comparability of experimental results.
    • HRP-conjugated IgG antibody(1 x 12ml) --Bind to the anti-HEV IgG antibody, and HRP catalyzes the TMB to elicit a chromogenic reaction.
    • Sample Diluent (1x 12ml) --Dilute the sample to an appropriate concentration.
    • Wash Buffer (20x concentrate) (1 x 50 ml) --Wash away unbound or free substances.
    • Substrate A (1 x 6ml) --Mix with substrate B and interact with HRP, eliciting a Chromogenic reaction.
    • Substrate B (1 x 6ml) --Mix with substrate A and interact with HRP, eliciting a Chromogenic reaction.
    • Stop Solution (1 x 6ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells)--Cover the microplate when incubation.
    • An Instruction manual

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  • 本试剂盒不含材料:
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 630 nm.
    • An incubator that can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 货期:
    3-5 working days

产品评价

相关问答

 常见问题解答
Q:

1.#CSB-E04811h,Human Hepatitis E virus antibody(IgG)ELISA Kit
2.#CSB-E04812hHuman Hepatitis E virus antibody(IgM)ELISA Kit what is the difference between 1 and 2? we need for human use. do these have ce?

A:
Thanks for your inquiry. CSB-E04811h is used to detect IgG and CSB-E04812h is used to detect IgM. I will send you the kit manuals by email.
As our elisa kits are only for research purpose,not for diagnosis,we did not go to get CE certificate.