Human Interleukin 35(IL-35) ELISA Kit 酶联免疫试剂盒-武汉华美生物

Human Interleukin 35(IL-35) ELISA Kit

产品详情

产品描述：

人白介素35(IL-35)酶联免疫试剂盒（CSB-E13126h）为双抗夹心法ELISA试剂盒，定量检测血清、组织培养上清液、尿液、组织匀浆样本中的IL-35含量。IL-35是一种具有重要生物学意义的靶点。它是一种免疫抑制性细胞因子。研究机制方面，IL-35可调节免疫细胞功能，抑制炎症反应。在多种疾病背景下，如自身免疫病、肿瘤等，对其深入研究有望为开发新的治疗策略提供方向。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于科研领域中对IL - 35表达水平的定量分析，例如探索炎症性疾病、自身免疫疾病模型中的免疫调控机制，或研究肿瘤微环境中免疫抑制性细胞因子的动态变化，为研究IL - 35在免疫稳态维持、慢性炎症及肿瘤免疫逃逸中的作用提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

IL-35
种属：

Homo sapiens (Human)
样本类型：

serum, cell culture supernates, urine, tissue homogenates
检测范围：

62.5 pg/mL-4000 pg/mL
灵敏度：

15.6 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human IL-35 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-98
1:2	Average %	98
	Range %	94-103
1:4	Average %	97
	Range %	90-106
1:8	Average %	94
	Range %	88-109

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

引用文献

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.