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中文名称：人白介素17B (IL17B)酶联免疫试剂盒
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货号：CSB-E15918h
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规格：96T/48T
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价格：￥3600/￥2500
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其他：
产品详情
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产品描述：CUSABIO人白介素17B（IL17B）酶联免疫检测试剂盒（货号：CSB-E15918h），采用双抗体夹心法定量检测血清、血浆及组织匀浆样本中IL17B蛋白的表达水平。IL17B属于IL-17细胞因子家族成员，参与调控炎症反应、宿主防御及组织修复过程，其表达水平变化与多种免疫相关病理机制研究密切相关。试剂盒检测灵敏度为31.25 pg/ml，线性检测范围覆盖31.25-2000 pg/ml，可在标准曲线范围内实现精准定量分析，适用于基础科研中探索IL17B在免疫调控、炎症性疾病模型或肿瘤微环境中的作用机制，也可用于体外药物筛选实验中生物标志物的动态监测。实验操作采用预包被特异性捕获抗体的96孔板，通过生物素标记检测抗体与链霉亲和素-HRP系统显色，配套标准品及优化缓冲液确保检测稳定性和特异性，样本类型兼容性广泛，满足不同实验设计的科研需求，仅限科学研究使用。
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别名：Cytokine like protein Zcyto7 ELISA Kit; Cytokine Zcyto7 ELISA Kit; IL 17B ELISA Kit; IL 20 ELISA Kit; IL-17B ELISA Kit; IL-20 ELISA Kit; IL17B ELISA Kit; IL17B_HUMAN ELISA Kit; IL20 ELISA Kit; Interleukin 17 beta ELISA Kit; Interleukin 17B ELISA Kit; Interleukin 20 ELISA Kit; Interleukin-17B ELISA Kit; Interleukin-20 ELISA Kit; MGC138900 ELISA Kit; MGC138901 ELISA Kit; Neuronal interleukin 17 related factor ELISA Kit; Neuronal interleukin-17-related factor ELISA Kit; NIRF ELISA Kit; ZCYTO7 ELISA Kit
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缩写：
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Uniprot No.：
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种属：Homo sapiens (Human)
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样本类型：serum, plasma, tissue homogenates
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检测范围：31.25 pg/ml-2000 pg/ml.
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灵敏度：7.8 pg/mL
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Immunology
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测定原理：quantitative
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测定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean(pg/ml)
252.019
260.431
227.817
252.542
239.054
254.114
SD
0.034
0.028
0.032
0.045
0.049
0.042
CV(%)
5.015
4.035
5.071
6.627
7.504
6.158
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线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human IL17B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
106
Range %
103-109
1:2
Average %
93
Range %
92-98
1:4
Average %
92
Range %
86-96
1:8
Average %
94
Range %
89-97
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回收率：
The recovery of human IL17B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
103
99-109
EDTA plasma (n=4)
92
87-97
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标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml
OD1
OD2
Average
Corrected
2000
2.066
2.122
2.094
1.974
1000
1.574
1.625
1.600
1.480
500
1.082
1.113
1.098
0.978
250
0.643
0.687
0.665
0.545
125
0.391
0.406
0.399
0.279
62.50
0.256
0.255
0.256
0.136
31.25
0.211
0.221
0.216
0.096
0
0.119
0.121
0.120
LOD
14.8pg/ml
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数据处理：
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货期：3-5 working days
引用文献
- Anti-metastatic effect and mechanisms of Wenshen Zhuanggu Formula in human breast cancer cells. Han XH.et al,J Ethnopharmacol.,2015
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靶点详情
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功能：Stimulates the release of tumor necrosis factor alpha and IL-1-beta from the monocytic cell line THP-1.
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基因功能参考文献：
- The results indicate that the IL-17B/IL-17RB signaling can promote the growth and migration of tumor cells. PMID: 27146881
- Our data revealed a new mechanism that IL-17B enhanced the progression of gastric cancer by activating mesenchymal stem cells. PMID: 28145881
- Segregation analysis revealed that variants c.475T>G in SKP1, c.671G>A in PROB1, and c.527G>A in IL17B in the 5q31.1-q35.3 linkage region, and c.850G>A in HKDC1 in the 10q22 locus completely segregated with the phenotype in the studied Keratoconus family PMID: 27703147
- This comprehensive review details the recognition of activity, signaling, and the roles of IL17B-IL17RB in breast cancer have caused to determination of new therapeutic mechanisms--{REVIEW} PMID: 28160754
- Studies indicate that interleukin 17 (IL-17B)/interleukin 17 receptor B (IL-17RB) signaling is essential for pancreatic cancer malignancy. PMID: 26285835
- IL-17B-IL-17RB signaling promotes pancreatic cancer malignancy. PMID: 25732306
- Results show that amplified signaling of interleukin-17 receptor B (IL-17RB) and its ligand IL-17B promot tumorigenicity in breast cancer cells and impeded acinus formation in immortalized normal mammary epithelial cells. PMID: 23851503
- IL-17B can enhance the effects of TNF-alpha on the production of cytokines and chemokines that control immune cell trafficking and neutrophil homeostasis in the inflamed tissues. PMID: 24056520
- IL-17A, IL-17B, IL-17F and IL-23 in systemic lupus erythematosus patients were examined and the correlation between levels of the investigated cytokines and VEGF, PIGF, as well as number of endothelial cells, was investigated. PMID: 23661335
- expression and functional study of IL-17B PMID: 22394632
- IL-17 may play an important role in the occurrence of nasal polyps by overexpression. PMID: 16805377
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亚细胞定位：Secreted.
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蛋白家族：IL-17 family
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组织特异性：Expressed in adult pancreas, small intestine, stomach, spinal cord and testis. Less pronounced expression in prostate, colon mucosal lining, and ovary.
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数据库链接：
