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  4. Human Kallikrein 2(hK 2) ELISA Kit

Human Kallikrein 2(hK 2) ELISA Kit

说明书下载
  • 中文名称：
    人激肽释放酶2(hK 2)酶联免疫试剂盒
  • 货号：
    CSB-E13094h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：
    人激肽释放酶2（hK 2）是一种丝氨酸蛋白酶，属于激肽释放酶家族成员，主要在前列腺组织中表达，参与细胞外基质的降解及激素调控过程，其表达水平与前列腺疾病的发生发展密切相关，是肿瘤微环境及分子机制研究的重要靶点之一。本试剂盒（货号：CSB-E13094h）采用双抗体夹心法（Sandwich ELISA）定量检测人血清、血浆及组织匀浆样本中的hK 2蛋白，检测范围覆盖62.5 pg/mL至4000 pg/mL，具备高灵敏度和宽泛的线性检测区间，适用于低浓度样本的精准分析。试剂盒包含预包被特异性抗体的微孔板、标准品、检测抗体及显色系统等核心组分，操作流程标准化，可在4-6小时内完成检测。科研领域可将其应用于前列腺癌相关分子机制研究、疾病标志物筛选、药物疗效评估或病理模型分析等方向，例如通过检测患者体液或组织样本中hK 2的动态变化，探索其与肿瘤侵袭性、转移潜能等生物学行为的关联性，为癌症诊疗的科研探索提供可靠工具。
  • 别名：
    Glandular kallikrein 2 ELISA Kit; Glandular kallikrein-1 ELISA Kit; hGK 1 ELISA Kit; hGK-1 ELISA Kit; hK2 ELISA Kit; Kallikrein 2 prostatic ELISA Kit; Kallikrein related peptidase 2 ELISA Kit; kallikrein, glandular ELISA Kit; Kallikrein, prostatic ELISA Kit; Kallikrein-2 ELISA Kit; KLK 2 ELISA Kit; Klk-2 ELISA Kit; Klk2 ELISA Kit; KLK2_HUMAN ELISA Kit; KLK2A 2 ELISA Kit; KLK2A2 ELISA Kit; MGC12201 ELISA Kit; Tissue kallikrein 2 ELISA Kit; Tissue kallikrein-2 ELISA Kit; Ton ELISA Kit
  • 缩写：
    KLK2
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    62.5 pg/mL-4000 pg/mL
  • 灵敏度：
    15.6 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human hK 2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 87
    Range % 82-91
    1:2 Average % 94
    Range % 89-98
    1:4 Average % 97
    Range % 94-107
    1:8 Average % 92
    Range % 88-96
  • 回收率：
    The recovery of human hK 2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 91-99
    EDTA plasma (n=4) 103 98-108
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    4000 2.371 2.201 2.286 2.180
    2000 1.973 1.921 1.947 1.841
    1000 1.312 1.405 1.359 1.253
    500 0.712 0.732 0.722 0.616
    250 0.337 0.342 0.340 0.234
    125 0.256 0.261 0.259 0.153
    62.5 0.197 0.199 0.198 0.092
    0 0.105 0.107 0.106 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

产品评价

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相关问答

■ 常见问题解答
Q:

Please provide the following information regarding Human Kallikrein 2, hK 2 ELISA Kit (CSB-E13094h)
1.What is the standard? (Recombinant full-length protein? Partial protein
(range?)?, peptide (sequence range?), native protein?)
2. If recombinant protein, then what is the host?
3. Is the capture antibody a monoclonal or polyclonal?
4. What is the host of the capture antibody?
5. Is the detection antibody a monoclonal or polyclonal?
6. What is the host of the detection antibody?

A:
Thanks for your inquiry.
CSB-E13094h
This kit is designed against full length protein.
Standard is 293 cell-derived recombinant protein.
Capture antibody is mouse monoclonal antibody.
Detection antibody is goat polyclonal antibody.
Pls let me know if you have any further questions. Thank you.

靶点详情

  • 功能：
    Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
  • 基因功能参考文献：
    1. glycosylation changes the enzymatic activity of KLK2 in a drastically substrate-dependent manner. PMID: 26582203
    2. The results indicated that W-hK2 had a defect in cellular trafficking due to its misfolding and that it activated the unfolded protein response, suggesting a mechanism to explain the association of the T allele with higher prostate cancer risk. PMID: 25847286
    3. miR-378 was predicted to target both KLK2 and KLK4 and downregulated levels detected in prostate cancer patients. PMID: 25153390
    4. The differential regulation of alternative transcripts (using KLK2, KLK3 and KLK4 as models) by androgens and anti-androgens as an indicator of prostate cancers, was investigated. PMID: 25153393
    5. Structure-function analyses of KLK2 establish the 99-loop as master regulator of its activity. PMID: 25326387
    6. Predictions based on levels of four kallikrein markers, including KLK2, in blood distinguish between pathologically insignificant and aggressive disease after radical prostatectomy with good accuracy. PMID: 23683475
    7. Alteration of cellular junctions in benign prostatic hyperplasia could contribute to the presence of luminal epithelial secreted proteins prostate specific antigen (PSA)2 and and KLK2 in the stromal compartment. PMID: 24711254
    8. we present the first evidence that KLK2 can also function as an androgen receptor modulator that may modulate cell growth after the development of castration-resistant prostate cancer PMID: 24122203
    9. Associations observed in young, healthy men between the seminal plasma and serum concentrations of hK2 and PSA and several genetic variants in KLK2 and KLK3 could be useful to refine models of PSA cutoff values in prostate cancer testing. PMID: 24270797
    10. Genetic variants at ATF7IP and KLK2 contribute to the variance of %fPSA. PMID: 23359319
    11. Two SNPs, in beta-microseminoprotein at and in kallikrein-related peptidase 2 at, are associated with PCA3 score at genome-wide significance level PMID: 23555189
    12. TK promotes vessel growth by increasing the number of EPCs and enhancing their functional properties through the kinin B(2) receptor-Akt signaling pathway. PMID: 22435954
    13. An exploratory study of a KLK2 polymorphism as a prognostic marker in prostate cancer was found to be less likely associated with low Gleason score morphology. PMID: 21178268
    14. we identifiedand genotyped novel single-nucleotide polymorphisms in cancer cases and controls which verified prior associations in KLK2 and in MSMB (but not in KLK3) with prostate cancer PMID: 20424135
    15. Data show six non-synonymous amino acid or frame shift changes in the KLK3 gene and three changes in each of the neighboring genes, KLK15 and KLK2. PMID: 19823874
    16. The identification of unusual mRNA splice variants of the KLK2 and KLK3 genes that result from inclusion of intronic sequences adjacent to the first exon. PMID: 11834722
    17. Characterization of androgen receptor and nuclear receptor co-regulator expression in human breast cancer cell lines exhibiting differential regulation of kallikreins 2 and 3. PMID: 12124798
    18. kallikrein expression in nipple aspirate fluid- ethnic variation PMID: 12209605
    19. Measurements of free prostate specific antigen and hK2 improve on our ability to counsel patients prior to treatment as to their risk of biochemical recurrence PMID: 16152616
    20. An additional serum marker for the detection of prostatic cancer. PMID: 16388506
    21. a role for the KLK2 gene in prostate cancer susceptibility PMID: 17085659
    22. results imply that prostate cancer risk is associated with androgen receptor(AR)-CAG repeat and kallikrein-2 polymorphisms in Indian population but no unambiguous association was observed with PSA and AR-GGN repeat polymorphism PMID: 17257635
    23. role of kallikrein gene 2 and 3 variant SNPs in the etiology of prostate cancer among men of European ancestry PMID: 17593395
    24. %fPSA and hK2 add important predictive value in older men and much closer to diagnosis PMID: 17657743
    25. The two novel ETV4 fusion partners possess as predominant common characteristics androgen-induction and prostate-specific expression. PMID: 18451133
    26. Recombinant hK2 activates ERK1/2 signaling of prostate cancer cell lines, which express both PAR1 and PAR2. PMID: 18567807
    27. adding free PSA and human kallikrein 2 to standard pretreatment risk-assessment models to predict biochemical recurrence after radical prostatectomy for prostate cancer enhanced the AUC PMID: 19003994
    28. PSA and kallikrein 2 transcripts in the peripheral blood of prostate cancer patients during barchytherapy could serve as a predictor of biochemiacl outcome. PMID: 19434652
    29. Observational study of gene-disease association, gene-gene interaction, and genetic testing. (HuGE Navigator) PMID: 19223501

    收起更多

  • 蛋白家族：
    Peptidase S1 family, Kallikrein subfamily
  • 数据库链接：

    HGNC: 6363

    OMIM: 147960

    KEGG: hsa:3817

    STRING: 9606.ENSP00000313581

    UniGene: Hs.515560

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