Human Mast Cell Carboxypeptidase ELISA Kit
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中文名称：
人肥大细胞类羧肽酶酶联免疫试剂盒
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货号：
CSB-E13756h
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规格：
96T/48T
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价格：
￥3600/￥2500
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其他：
产品详情
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产品描述：
CUSABIO人肥大细胞类羧肽酶（CPA3）酶联免疫检测试剂盒（货号：CSB-E13756h），采用双抗体夹心法定量检测血清、血浆及组织匀浆样本中的CPA3蛋白含量。CPA3是肥大细胞特异性分泌的羧肽酶家族成员，在炎症反应、组织重塑及过敏性疾病中发挥重要作用，其表达水平与肥大细胞活化状态密切相关。本试剂盒检测灵敏度为0.312 ng/mL，标准曲线覆盖0.312-20 ng/mL的线性范围，可精准检测样本中低浓度目标蛋白。试剂盒包含预包被捕获抗体的96孔板、检测抗体及标准品，操作流程优化后可在4.5小时内完成检测，适用于科研领域中对肥大细胞相关疾病机制的研究，如过敏性疾病模型构建、炎症标志物分析以及药物干预下CPA3表达水平变化的评估，为探索肥大细胞功能调控提供可靠工具。
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别名：
carboxypeptidase A ELISA Kit; carboxypeptidase A3 (mast cell) ELISA Kit; Carboxypeptidase A3 ELISA Kit; carboxypeptidase A3 mast cell ELISA Kit; CBPA3_HUMAN ELISA Kit; Cpa3 ELISA Kit; Mast cell carboxypeptidase A ELISA Kit; mast cell carboxypeptidase A3 ELISA Kit; MC CPA ELISA Kit; MC-CPA ELISA Kit; tissue carboxypeptidase A ELISA Kit
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缩写：
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Uniprot No.：
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种属：
Homo sapiens (Human)
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样本类型：
serum, plasma, tissue homogenates
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检测范围：
0.312 ng/mL-20 ng/mL
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灵敏度：
0.078 ng/mL
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反应时间：
1-5h
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样本体积：
50-100ul
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检测波长：
450 nm
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研究领域：
Cell Biology
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测定原理：
quantitative
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测定方法：
Sandwich
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精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MC-CPA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 86 | |
| Range % | 80-92 | |
| 1:2 | Average % | 100 | |
| Range % | 94-103 | |
| 1:4 | Average % | 97 | |
| Range % | 94-100 | |
| 1:8 | Average % | 94 | |
| Range % | 86-98 | |
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回收率：
| The recovery of human MC-CPA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 94 | 89-98 | |
| EDTA plasma (n=4) | 96 | 94-98 | |
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标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | ng/ml | OD1 | OD2 | Average | Corrected | | | 20 | 2.477 | 2.453 | 2.465 | 2.366 | | | 10 | 1.993 | 1.924 | 1.959 | 1.860 | | | 5 | 1.280 | 1.271 | 1.276 | 1.177 | | | 2.5 | 0.685 | 0.678 | 0.682 | 0.583 | | | 1.25 | 0.433 | 0.424 | 0.429 | 0.330 | | | 0.625 | 0.286 | 0.269 | 0.278 | 0.179 | | | 0.312 | 0.190 | 0.179 | 0.185 | 0.086 | | | 0 | 0.101 | 0.097 | 0.099 | | |
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数据处理：
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货期：
3-5 working days
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