Human Myostatin,MSTN ELISA Kit 酶联免疫试剂盒-武汉华美生物

Human Myostatin,MSTN ELISA Kit

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产品描述：

人肌抑素（MSTN）是一种肌肉生长抑制因子，属于TGF-β超家族成员，通过调控肌肉细胞增殖与分化在肌肉发育和代谢平衡中发挥关键作用。本试剂盒（货号：CSB-E11300h）基于双抗体夹心法原理设计，能够对血清、血浆及组织匀浆样本中的MSTN蛋白进行定量检测，其线性检测范围为0.625-20 ng/mL，可精准识别低浓度目标分子。试剂盒包含预包被特异性抗体的酶标板、标准品、检测抗体及显色底物等核心组分，实验全程无需额外优化，操作简便且重复性良好。该产品适用于探究肌肉发育机制、运动代谢调控、肌肉萎缩相关疾病模型等基础研究领域，尤其可为运动科学、肌肉再生及代谢性疾病（如肥胖、Ⅱ型糖尿病）的分子机制研究提供可靠检测工具。通过检测不同生理或病理条件下生物样本中MSTN的浓度变化，研究人员可深入解析其在肌肉-脂肪代谢网络中的动态调控作用。
别名：

Cmpt ELISA Kit; GDF-8 ELISA Kit; GDF8_HUMAN ELISA Kit; Growth/differentiation factor 8 ELISA Kit; MSLHP ELISA Kit; MSTN ELISA Kit; MSTN myostatin ELISA Kit; Myostatin ELISA Kit; Myostatin Propeptide ELISA Kit
缩写：

MSTN
Uniprot No.：

O14793
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.625 ng/mL-20 ng/mL
灵敏度：

0.312 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human MSTN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	92-99
1:2	Average %	94
	Range %	91-98
1:4	Average %	101
	Range %	97-105
1:8	Average %	86
	Range %	82-90

回收率：

The recovery of human MSTN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-97
EDTA plasma (n=4)	86	82-91

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	1.851	1.992	1.922	1.878
10	1.058	1.136	1.097	1.053
4.375	0.502	0.524	0.513	0.469
1.875	0.268	0.255	0.262	0.218
0.625	0.116	0.119	0.118	0.074
0	0.043	0.045	0.044

本试剂盒所含材料：
- A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human MSTN antibody.
- Six vials standard (5 ml/bottle) ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial HRP-conjugated MSTN antibody (6 ml/bottle) ---Bind to the MSTN in the samples or standards and react with the substrate to make the solution chromogenic.
- Substrate A (1 x 7 ml)
- Substrate B (1 x 7 ml)
- One vial Stop Solution (7ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) ---Cover the microplate when incubation.
- An instruction manual
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本试剂盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm or 630 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

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功能：

Acts specifically as a negative regulator of skeletal muscle growth.
基因功能参考文献：
1. Mstn deficiency-induced apoptosis took place along with generation of reactive oxygen species and elevated fatty acid oxidation, which may play a role in triggering mitochondrial membrane depolarization, the release of cytochrome c, and caspase activation. PMID: 30241032
2. the maturation and secretion of myostatin precursor MstnPP and its metabolites in a human muscle cell line, was investigated. PMID: 29546591
3. Acute high-intensity interval exercise decreased irisin levels and increased myostatin levels. PMID: 29558345
4. Autosomal Dominant Polycystic Kidney Disease patients with moderately preserved renal function have higher levels of FasL, myostatin and urine TGF-beta1 than controls PMID: 29794429
5. Tolloid cleavage activates latent GDF8 by destabilizing specific prodomain-growth factor interfaces and primes the growth factor for release from the prodomain. PMID: 29343545
6. Myostatin pathway is down-regulated in the neuromuscular diseases. PMID: 29192144
7. the prodomain:GDF8 complex can exist in a fully latent state and an activated or "triggered" state where the prodomain remains in complex with the mature domain PMID: 29348202
8. Higher serum myostatin levels correlated with muscle mass loss, hyperammonemia, and impaired protein synthesis, as reflected by lower serum albumin levels and lower branched-chain amino acid to tyrosine ratio levels. High serum myostatin levels were also associated with a reduced OS rate in LC patients. PMID: 28627027
9. Study showed the expression of myostatin in healthy endometrium and a higher expression in endometriosis and endometrial cancer, suggesting myostatin involvement in human endometrial physiology and related pathologies. PMID: 28345488
10. Studied levels of myostatin in both serum and synovial fluid in patients with knee osteoarthritis and found both correlated with severity of knee osteoarthritis. PMID: 27878995
11. Myostatin (and Smad2) were significantly up-regulated in the failing heart of female patients, but not male patients. PMID: 28465115
12. The Growth Differentiation Factor 11 (GDF11) and Myostatin (MSTN) in tissue specific aging. PMID: 28472635
13. MSTN 153Arg(R) polymorphism is associated with long distance running success. PMID: 28007336
14. GDF8 promotes ovarian cancer cell migration via ALK4/5-SMAD2/3-E-cadherin signaling. PMID: 27481097
15. Results demonstrat that GDF8 stimulates the expression and secretion of CTGF in human granulosa cells and provide evidence that both proteins may play critical roles in the regulation of extracellular matrix formation in these cells. PMID: 27392496
16. These studies identify distinctive structural features of GDF11 that enhance its potency, relative to GDF8; however, the biological consequences of these differences remain to be determined. PMID: 28257634
17. Serum myostatin levels were significantly decreased in heart failure patients and associated with lower extremity muscle wasting. PMID: 27390974
18. our data showed a virtual absence of the variant (K) allele in MSTN rs1805086 in Japanese population, and no differences in allele/genotype frequencies in ACTN3 rs1815739 among centenarians and healthy controls of this country. PMID: 27861536
19. MSTN, but not GDF11, declines in healthy men throughout aging. PMID: 27304512
20. Multivariate regression analysis revealed that myostatin levels correlated significantly with tricuspid annular plane systolic excursion values and right ventricle myocardial performance index among the study patients PMID: 27323660
21. Study measured circulating myostatin levels in seven inherited muscle diseases using an immunoaffinity LC-MS/MS approach, found significantly lower serum myostatin concentrations in numerous muscle disease patient populations and the associations with clinical measurements suggests the potential utility of myostatin as a biomarker of genetic muscle disease progression PMID: 28074267
22. data indicated that serum myostatin concentration did not correlate with muscle and bone mass in postmenopausal women PMID: 27144806
23. Myostatin mRNA expression in skeletal muscle was significantly reduced compared with pre-exercise values at all time points with no difference between exercise intensity. PMID: 27467217
24. Low expression of serum MSTN is associated with Cachexia Prevention in Patients with Medullary Thyroid Cancer. PMID: 27165248
25. Myostatin was differentially expressed in the muscle and adipose tissue in relation to physical activity and dysglycaemia PMID: 26572800
26. Our results suggest that serum levels of myostatin and irisin are related in patients with type 2 diabetes PMID: 26438394
27. Matrix metalloproteinase 14 was highly expressed in uterine leiomyoma and correlated with myostatin and activin A mRNA expression. Moreover, MMP14 and myostatin mRNA expression correlated significantly and directly with the intensity of dysmenorrhea. PMID: 26138721
28. A synthetic peptide corresponding to this decorin region dose-dependently inhibited the response to myostatin in cardiomyocytes PMID: 27559042
29. Findings suggest that GDF8 and CTGF may play critical roles in the regulation of proliferative events in human granulosa cells. PMID: 26577677
30. Myostatin concentrations in plasma and protein expression in placental tissue are significantly higher in women with preeclampsia. Cytokine production by first-trimester placental tissues was altered following myostatin treatment. PMID: 25736326
31. Myostatin is a well-described negative regulator of postnatal skeletal and cardiac muscle mass and modulates metabolic processes. It functions in the heart, skeletal muscle, and brain. Review. PMID: 27034275
32. Plasma myostatin levels are increased in chronic obstructive pulmonary disease patients who have cor pulmonale. PMID: 26998756
33. In human granulosa cells, GDF8 may play an important role in the modulation of cellular responsiveness to gonadotropins and in the regulation of ovarian steroid production, most likely as a luteinization inhibitor. PMID: 26607022
34. Plasma myostatin might be suitable in predicting weight regain after marked weight loss, but no association with weight loss was observed in patients undergoing a non-surgical weight loss program. PMID: 26393401
35. Plasma MSTN level was elevated in an early stage of CKD, which could be involved in the progression of sarcopenia. PMID: 26502079
36. it is becoming clearer that besides its conventional role in muscle, myostatin plays a critical role in metabolism. Hence, molecular mechanisms by which myostatin regulates several key metabolic processes need to be further explored. PMID: 26305594
37. The aim of this study was to investigate MSTN polymorphisms in an elderly sarcopenic population in Turkey and determine how they relate to sarcopenia. PMID: 26046327
38. There is a significant decrease in levels of circulating myostatin in postsurgical acute phase reaction. PMID: 25749570
39. Myostatin myocardial expression increases in the presence of structural cardiomyopathy either of hypertensive or other origin. PMID: 25915890
40. In contrast to elite endurance and power track and field athletes, the MSTN 153RR genotype was not found in short distance-swimmers, and among the long distance-swimmers it was not associated with top level swimming performance. PMID: 25936293
41. Lower serum myostatin independently associated with MetS, central obesity, low HDL-C, and high triglycerides after adjustment. Higher serum myostatin is associated with favorable metabolic profiles. PMID: 25254550
42. Results indicate that GDF8 down-regulates PTX3 expression via SMAD-dependent signaling in human granulosa cells suggesting a potential role for GDF8 in the regulation of follicular function. PMID: 25641196
43. Adenomyotic tissues express high levels of myostatin, follistatin, and activin type II receptors. PMID: 26086422
44. This is the first demonstration of a spatial asymmetry in the expression pattern of Mstn/IGF-I in healthy hearts, which is likely to play a role in the different growth regulation of left vs. right ventricle PMID: 25591711
45. Myostatin expression in placental tissue is altered under stress conditions (e.g. obesity and abnormal glucose metabolism) found in pregnancies complicated with gestational diabetes mellitus. PMID: 25443639
46. we show that the K153R mutation significantly increases the rate of proteolysis of promyostatin by furin, but has no effect on the activity of the latent complex or the cleavage of the latent complex by bone morphogenetic protein 1 (BMP-1). PMID: 25543063
47. MyoD acts to promote SC proliferation and transition of cells into differentiation, while myogenin is known to drive terminal differentiation PMID: 25108351
48. role of myostatin in cardiovascular disease and cachexia PMID: 24680839
49. Myostatin localization was positively identified in extravillous trophoblast. Myostatin positively affected proliferation and migration of extravillous trophoblast. PMID: 25093622
50. The present study demonstrated that the variant alleles of MSTN A55T and K153R polymorphisms could significantly enhance muscle hypertrophy in response to strength training among Han Chinese men. PMID: 24479661
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相关疾病：

Muscle hypertrophy (MSLHP)
亚细胞定位：

Secreted.
蛋白家族：

TGF-beta family
数据库链接：

HGNC: 4223

OMIM: 601788

KEGG: hsa:2660

STRING: 9606.ENSP00000260950

UniGene: Hs.41565

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.