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  4. SARS-CoV-2 Neutralizing Antibody ELISA Kit

SARS-CoV-2 Neutralizing Antibody ELISA Kit

文献引用 (2) 说明书下载
  • 中文名称：
    新型冠状病毒(SARS-CoV-2)中和抗体酶联免疫试剂盒
  • 货号：
    CSB-EL33243HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    新型冠状病毒(SARS-CoV-2)中和抗体酶联免疫试剂盒（CSB-EL33243HU）为竞争法ELISA试剂盒，定性检测血清、血浆样本中的SARS-CoV-2 neutralizing Ab含量。SARS-CoV-2 neutralizing Ab即新冠病毒中和抗体。背景上，新冠疫情全球大流行促使其研究。机制方面，中和抗体可识别并结合新冠病毒刺突蛋白，阻止病毒与宿主细胞受体结合，阻断感染过程，是抗病毒研究关键方向。为疫苗研发、免疫应答评估及感染康复者抗体动态研究提供重要依据；适用于科研场景中疫苗接种后抗体效价追踪、群体免疫水平研究或病毒感染动物模型的免疫效果评估本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2
  • 缩写：
    SARS-CoV-2 neutralizing Ab
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Infectious Diseases
  • 测定原理：
    qualitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml OD1 OD2 Average
    10000 0.099 0.092 0.096
    5000 0.145 0.136 0.141
    2500 0.194 0.183 0.189
    1250 0.274 0.263 0.269
    625 0.344 0.371 0.358
    312 0.519 0.528 0.524
    156 0.901 0.907 0.904
    78 1.509 1.412 1.461
    39 1.787 1.723 1.755
    19.5 2.121 1.958 2.040
    9.75 2.233 2.138 2.186
    0 2.394 2.328 2.361
  • 本试剂盒所含材料：
      • One 96-well Microtiter plate ( coated Microwells) -- This microwell plate has been pre-coated with recombinant SARS-CoV-2 S RBD antigen and can be dismountable into 12 strips of 8 wells.
      • A bottle of Standard (10 x concentrate; 200 μl/vial) -- Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
      • A bottle of HRP-conjugated ACE2 (100 x concentrate; 60 μl/vial) -- ACE2 interacts with SARS-CoV-2 S RBD, mimicking the process of SARS-CoV-2 entry into the host cells. HRP catalyzes the TMB Substrate solution, eliciting a chromogenic reaction.
      • A bottle of HRP-conjugate Diluent (10 ml/vial) -- Dilute the HRP-conjugated ACE2 solution.
      • Two bottles of Sample Diluent (20 ml/vial) -- Reconstitute the standard and dilute the sample to an appropriate concentration.
      • A bottle of Wash Buffer (25 x concentrate; 20 ml/vial) -- Wash away unbound or free substances.
      • A bottle of TMB Substrate (10 ml/vial) -- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • A bottle of Stop Solution (10 ml/vial) -- Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) -- Seal the plate when incubation.
      • One Instruction manual

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  • 本试剂盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer.
      • Absorbent paper for blotting the microtiter plate.
      • 50-300ul multi-channel micropipette
      • 100ml and 500ml graduated cylinders.
      • Deionized or distilled water.
      • Pipette tips
      • Timer
      • Test tubes for dilution.

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  • 货期：
    3-5 working days

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相关问答

■ 常见问题解答
Q:

What is the assay procedure?

A:

1. Prepare all reagents, samples, and working standards as instructed.
2. Refer to the Assay Layout Sheet to calculate the number of the wells (including a blank well) to be used and put the remaining wells and the desiccant back into the pouch to seal and store at 4 °C.
3. Add 50 µl of standard or sample (may require dilution) to each well, followed by 50 µl of HRP-conjugated ACE2 (not to the blank well). Shake the plate gently for 1 minute. Seal and incubate for 1 hour at  37 °C.
4. After thorough washing, add 90 μl of TMB Substrate Solution to each well. Incubate for 20 minutes at 37°C.
5. Add 50 µl Stop Solution to each well and gently tap the plate to ensure thorough mixing.
6. Read the OD (optical density) value at 450 nm within 5 minutes using a microplate reader.

Q:

What is the principle of the SARS-CoV-2 Neutralizing Antibody ELISA Kit?

A:
This assay is based on competitive method. The kit detects circulating neutralizing antibodies against SARS-CoV-2 that block the interaction between the receptor binding domain (RBD) of the viral spike glycoprotein with the ACE2 cell surface receptor.
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