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Human SARS-CoV-2 N IgG Antibody ELISA Kit

  • 中文名称:
    人新型冠状病毒核蛋白(SARS-CoV-2 N) IgG抗体酶联免疫试剂盒
  • 货号:
    CSB-EL3325HU
  • 规格:
    96T/48T
  • 价格:
    ¥3600/¥2500
  • 其他:

产品详情

  • 产品描述:
    人新型冠状病毒核蛋白(SARS-CoV-2 N) IgG抗体酶联免疫试剂盒(CSB-EL3325HU)为间接法ELISA试剂盒,定性检测血清、血浆样本中的SARS-CoV-2 N Ab (IgG)含量。SARS-CoV-2 N Ab (IgG)是针对新冠病毒核衣壳蛋白的IgG抗体靶点。新冠疫情促使对其深入研究。检测该抗体水平,可评估人体对病毒的免疫反应情况,为疫情监测、疫苗效果评估等提供重要依据。适用于科研场景中COVID - 19感染后抗体动态研究、疫苗免疫效果评估、人群血清流行病学调查等,为病毒免疫机制研究提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    N; Nucleoprotein; N; Nucleocapsid protein; NC; Protein N
  • 缩写:
    SARS-CoV-2 N Ab (IgG)
  • Uniprot No.:
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Infectious Diseases
  • 测定原理:
    qualitative
  • 测定方法:
    Indirect
  • 标准曲线:

    Test parameter

    specification

    test result

    Positive control

    >0.8

    0.967

    Negative control

    <0.2

    0.184

    Positive rate

    20,Positive

    100%

    Negative rate

    20,Negative

    100%

  • 本试剂盒所含材料:
      • A 96-well Coated assay plate --This microplate has been pre-coated with human SARS-CoV-2 nucleoprotein.
      • Negative Control (1 x 800 μl) --It is free of the SARS-CoV-2 N IgG antibody and used to preclude the false positive.
      • Positive Control (1 x 800 μl) --Used to evaluate the validity, stability, and comparability of the test results.
      • HRP-conjugated anti-Human IgG antibody (100 x concentrate) (1 x 120 μl) --Act as the detection antibody.
      • HRP-conjugate Diluent (1 x 20 ml) --Dilute the HRP-conjugated anti-Human IgG antibody.
      • Sample Diluent (2 x 20 ml) --Dilute the sample solution.
      • Wash Buffer (25 x concentrate) (1 x 20 ml) --Wash the unbound reagents.
      • TMB Substrate (1 x 10 ml) --React with HRP, eliciting a chromogenic color reaction.
      • Stop Solution (1 x 10 ml) --Stop the color reaction. The solution turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --Seal the microplate when incubation.
      • An Instruction manual

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  • 本试剂盒不含材料:
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer.
      • Absorbent paper for blotting the microtiter plate.
      • 50-300ul multi-channel micropipette
      • 100ml and 500ml graduated cylinders.
      • Deionized or distilled water.
      • Pipette tips
      • Timer
      • Test tubes for dilution.

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  • 货期:
    3-5 working days

产品评价

相关问答

 常见问题解答
Q:

如何使用检测核蛋白或刺突蛋白的Elisa试剂盒?推荐的使用目的有什么不同吗?另外,您建议对CoVID-19检测是用IgG还是IgM?

A:
关于检测CoVID-19的ELISA试剂盒,我们只能提供以下产品:CSB-EL3325HU, Human Novel Coronavirus Nucleoprotein (SARS-CoV-2 N) IgG Antibody ELISA Kit。它只用于科研,不用于临床或诊断。通常,IgM首先在原发性免疫反应中产生。IgM首先出现在病毒感染过程中,但持续时间不长;IgG是再免疫反应产生的主要抗体,持续时间较长,表明过去有感染。

靶点详情

  • 功能:
    Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host smad3.
  • 亚细胞定位:
    Virion. Host cytoplasm.
  • 蛋白家族:
    Betacoronavirus nucleocapsid protein family