Human Osteocalcin/Bone gla protein,OT/BGP ELISA kit 酶联免疫试剂盒-武汉华美生物

Human Osteocalcin/Bone gla protein,OT/BGP ELISA kit

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人骨钙素/骨谷氨酸蛋白(OT/BGP)酶联免疫试剂盒（CSB-E05128h）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的BGLAP含量。BGLAP（骨钙素）是一种由成骨细胞分泌的激素，参与骨骼重塑和能量代谢。其羧化形式负向调节骨形成，同时促进骨吸收和矿化，对磷灰石和钙有强亲和力。研究显示，BGLAP在骨骼发育、成骨细胞分化和生物矿化组织中发挥重要作用。试剂盒检测范围为31.25 pg/ml-2000 pg/ml，灵敏度为7.8 pg/ml。适用于骨代谢相关基础研究，如骨质疏松症动物模型评估、骨骼发育机制探索、骨组织工程材料生物活性分析等科研领域。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

BGLAP ELISA Kit; BGP ELISA Kit; Bone gamma carboxyglutamate (gla) protein ELISA Kit; Bone gamma carboxyglutamate gla protein osteocalcin ELISA Kit; Bone gamma carboxyglutamate protein ELISA Kit; Bone Gla protein ELISA Kit; Gamma carboxyglutamic acid containing protein ELISA Kit; Gamma-carboxyglutamic acid-containing protein ELISA Kit; OC ELISA Kit; OCN ELISA Kit; OSTCN_HUMAN ELISA Kit; Osteocalcin ELISA Kit; OTTHUMP00000016586 ELISA Kit; PMF1 ELISA Kit
缩写：

BGLAP
Uniprot No.：

P02818
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

31.25 pg/ml-2000 pg/ml
灵敏度：

7.8 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human OT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	98
	Range %	95-106
1:2	Average %	99
	Range %	93-105
1:4	Average %	94
	Range %	86-99
1:8	Average %	97
	Range %	91-105

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

2000

2.552

2.634

2.593

2.483

1000

1.764

1.667

1.716

1.605

500

1.242

1.228

1.235

1.125

250

0.747

0.729

0.738

0.628

125

0.476

0.451

0.463

0.353

62.5

0.324

0.333

0.329

0.218

31.25

0.194

0.184

0.189

0.079

0.111

0.109

0.110

本试剂盒所含材料：
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数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

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最新研究进展：

BGLAP (Bone Gla Protein or Osteocalcin) 是一种在骨细胞中高度表达的蛋白质，参与骨代谢和钙离子调节。近期的研究表明，BGLAP 可能也参与神经系统的调节和认知功能。BGLAP 被认为是一个骨特异性蛋白质，它参与了骨形成和骨质疏松症的发病机制。BGLAP 的水平可用于评估骨转换和疾病的进展。
功能：

Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
基因功能参考文献：
1. The results of this study suggest that serum osteocalcin is associated with the onset of puberty in girls. PMID: 30089739
2. The correlation between Mycobacterium avium subsp. paratuberculosis (MAP) infection and elevation of sera ucOC, reduction of active osteocalcin and increased calcium supports MAP infection role in Crohn's disease and complications with osteoporosis. PMID: 29638140
3. The results showed no significant correlation between osteocalcin HindIII gene polymorphism and bone mineral density (BMD) and therefore osteocalcin HindIII gene polymorphism may not have major effects on BMD variation in postmenopausal North Indian women. PMID: 30179426
4. Decreased circulating carboxylated osteocalcin is associated with low-energy bone fractures in children. PMID: 29882816
5. a decrease in serum osteocalcin levels is associated with future development of type 2 diabetes mellitus. PMID: 28766135
6. Findings from the available data indicated an overall significant inverse association between serum osteocalcin and body mass index--{REVIEW} PMID: 28822067
7. relatively lower serum osteocalcin levels were associated with a higher risk of MACE in Chinese men with CAD PMID: 28921598
8. undercarboxylated form of osteocalcin in serum not related significantly to semen parameters or measured hormones in male infertility PMID: 28000970
9. serum level not correlated with gender, race/ethnicity, age, diabetes type, BMI percentile, random C-peptide, glucose, or HbA1c at pediatric diabetes diagnosis; serum level inversely correlated with HbA1c at first office visit following diagnosis PMID: 28093839
10. Serum osteocalcin was directly and independently correlated with circulating uric acid in a population of healthy obese subjects. PMID: 28847266
11. gamma-carboxylation of OCN on Glu17 residue correlates with insulin resistance and subclinical inflammation, suggesting that gamma-carboxylation of OCN negatively regulates its endocrine action PMID: 28242268
12. There were differences in the associations between total osteocalcin and glucose metabolism in Han and Uygur T2DM patients, indicating genetic factors may play a role in modulating OC and glucose metabolism in different ethnic population. PMID: 28732499
13. The study demonstrated that undercarboxylated osteocalcin (ucOC) is related to 25(OH)D and adiponectin concentrations. Both ucOC and 25(OH)D were lower in overweight and obese adolescents. PMID: 28443718
14. Increased advanced glycation end products and protein oxidative damage markers in the course of obesity may contribute to decreased carboxylated osteocalcin serum level. PMID: 28841723
15. Progressive calcification of atherosclerotic plaques is accompanied by insignificant accumulation of calcitonin and osteoprotegerin, whereas the osteocalcin expression significantly increased during calcification. PMID: 28429221
16. Undercarboxylated osteocalcin reflects the reserve capacity of beta-cell function, such as the bolus insulin secretion ability in patients with type 2 diabetes. PMID: 27889949
17. XPS showed that similar amounts of hOC and dhOC were absorbed onto hydroxyapaptite and octacalcium phosphate surfaces, but ToF-SIMS detected some small differences in the amino acid fragment intensities on these surfaces for adsorbed hOC and dhOC. PMID: 28521505
18. Common polymorphism rs1800247 in osteocalcin gene was associated with hypertension and diastolic blood pressure levels. The association of rs1800247 with hypertension was affected by its interaction with HOMA-IR. PMID: 27251081
19. These findings indicate the undercarboxylated form of OC is not implicated in age-related changes in skeletal muscle or adipose tissue mass in older community-dwelling adults. PMID: 27604070
20. Demonstrate a positive association between osteocalcin, a factor known to regulate bone remodelling, with cognitive performance in older non-demented women. PMID: 27515675
21. Common genetic variants of the osteocalcin gene may not be a major contributor to variations in serum osteocalcin or BMD in postmenopausal and elderly Chinese women. PMID: 28002816
22. Several detected relations between OCN and metabolites were strongly linked to renal function, however, a number of associations remained significant after adjustment for renal function. Intermediates of proline catabolism were associated with OCN reflecting the implication in bone metabolism. PMID: 28922389
23. Low serum osteocalcin concentrations are associated with type 2 diabetes. PMID: 27061949
24. Decreased blood concentration of Carboxylated osteocalcin is associated with metabolic complications and inflammation. PMID: 27667744
25. Total and undercarboxylated osteocalcin levels were significantly correlated with insulin secretion and insulin resistance indices, but not adiponectin levels, in pregnant women. PMID: 26708046
26. Osteocalcin and Sex Hormone Binding Globulin Compete on a Specific Binding Site of GPRC6A. PMID: 27673554
27. Osteocalcin plays an essential role for the maturation of mineral species and modulates osteogenic differentiation of mesenchymal stromal cells (MSCs). PMID: 28106724
28. Serum osteocalcin levels in pediatric obstructive sleep apnea hypopnea syndrome patients were significantly lower than normal controls before adenotonsillectomy. PMID: 26938749
29. the relationship between serum osteocalcin and glucose metabolism parameters in liver transplantation recipients, was examined. PMID: 26743527
30. Reduced plasma Osteocalcin levels are associated with cardiovascular and metabolic diseases. PMID: 26863345
31. An overview of osteocalcin progress. PMID: 26747614
32. the BMP-2/Smad1/5/RUNX2 signaling pathway participates in the silicon-mediated induction of COL-1 and osteocalcin synth PMID: 27025722
33. Data indicate that lower osteocalcin was an independent predictor of worse cognitive performance on multivariate analysis. PMID: 26406918
34. A common polymorphism rs1800247 in osteocalcin gene may effect the risk of osteoporosis and fracture and serum osteocalcin levels in Chinese. PMID: 26194493
35. There is an inverse relationship between the visfatin and undercarboxylated osteocalcin in type 2 diabetes patients with diabetic kidney disease. PMID: 26922969
36. This study investigated osteocalcin, metabolic parameters and anthropometric characteristics in normal weight and overweight/obese children. PMID: 26772621
37. Nonalcoholic fatty liver disease negatively associated with right-hip bone mineral density and serum osteocalcin in Korean men. PMID: 26636404
38. The aims of this study were to evaluate OCN and sclerostin levels in subjects who underwent coronary artery bypass graft (CABG) surgery compared with those in normal controls. PMID: 26576474
39. serum level not associated with weight loss or body fat percentage PMID: 25963022
40. Serum osteocalcin levels are inversely correlated with nonalcoholic fatty liver disease in postmenopausal Chinese women with normal blood glucose levels. PMID: 26567728
41. Serum total osteocalcin level is significantly lower in 2 diabetes mellitus patients than controls. PMID: 26372899
42. Elevated serum preptin and decreased osteocalcin concentrations, together with insulin resistance, are associated with obesity and overweight. PMID: 25675353
43. gestational diabetes is not associated with osteocalcin, under-carboxylated osteocalin, osteopontin and leptin and does not correlate with insulin resistance. PMID: 25480426
44. Serum uOC levels in non-dialysis patients with CKD are significantly lower than those in healthy individuals, and uOC is closely associated with subclinical atherosclerosis in CKD patients. PMID: 26381729
45. Endocrine function of osteocalcin and the cell biology events that allow osteocalcin to become a hormone. [review] PMID: 25577163
46. Data indicate that higher osteocalcin (OC) or its undercarboxylated form (ucOC) concentration and ucOC to osteocalcin ratio (OCR) were associated with the risk of coronary calcification in men. PMID: 25868823
47. Data suggest that a low serum osteocalcin level was an independent risk factor for abdominal obesity. PMID: 25871789
48. Correlation between osteocalcin-positive endothelial progenitor cells and spotty calcification in patients with coronary artery disease PMID: 25644945
49. These findings unequivocally document that increased vitamin K intake reduces the uncarboxylated form of OCN PMID: 25855755
50. Serum osteocalcin level was not independently associated with C-IMT in a metabolically healthy Chinese population. PMID: 26077201
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亚细胞定位：

Secreted.
蛋白家族：

Osteocalcin/matrix Gla protein family
数据库链接：

HGNC: 1043

OMIM: 112260

KEGG: hsa:632

STRING: 9606.ENSP00000357255

UniGene: Hs.654541

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.