-
中文名称：人血小板衍生生长因子BB(PDGF-BB)酶联免疫试剂盒
-
货号：CSB-E08923h
-
规格：96T/48T
-
价格：￥3200/￥2500
-
其他：
产品详情
-
产品描述：本试剂盒（货号CSB-E08923h）采用双抗体夹心法原理，专为定量检测人血清、血浆及组织匀浆样本中的血小板衍生生长因子BB（PDGF-BB）而设计。PDGF-BB作为重要的细胞生长调控因子，参与血管生成、组织修复及细胞增殖等生理过程，其异常表达与肿瘤微环境、纤维化疾病等病理机制密切相关。试剂盒检测灵敏度达31.25 pg/mL，线性范围为31.25-2000 pg/mL，通过预包被高特异性抗体实现精准捕获目标蛋白，并采用酶标二抗进行化学信号放大，确保实验结果的稳定性和重复性。适用于体外细胞培养上清、动物模型组织样本或生物体液中的PDGF-BB水平检测，可为心血管疾病机制研究、抗纤维化药物开发、肿瘤微环境分析等基础科研提供定量数据支持，操作流程兼容常规实验室设备，96孔板设计满足高通量检测需求，配套标准品及反应缓冲液助力实验体系优化。本产品仅供科学研究使用，实验结果不可作为临床诊断依据。
-
缩写：PDGF-BB
-
种属：Homo sapiens (Human)
-
样本类型：serum, plasma, tissue homogenates
-
检测范围：31.25 pg/mL-2000 pg/mL
-
灵敏度：7.81 pg/mL
-
反应时间：1-5h
-
样本体积：50-100ul
-
检测波长：450 nm
-
研究领域：Cardiovascular
-
测定原理：quantitative
-
测定方法：Sandwich
-
精密度：
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess. -
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human PDGF-BB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
-
回收率：
The recovery of human PDGF-BB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
-
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
-
数据处理：
-
货期：3-5 working days
引用文献
- VALUTAZIONE DELL'UTILIZZO DEL LISATO PIASTRINICO (LP) IN MEDICINA RIGENERATIVA VETERINARIA EDE DELLA LOMBARDIA,/,2023
- The use of human‐derived feeder layers for the cultivation of transplantable human epidermal cell sheet to repair second degree burn wounds Z Mingqi,Skin research and technology,2023
- Platelet Activation Favours NOX2-Mediated Muscle Damage in Elite Athletes: The Role of Cocoa-Derived Polyphenols A D'Amico,Nutrients,2022
- Small-Dose Sunitinib Modulates p53, Bcl-2, STAT3, and ERK1/2 Pathways and Protects against Adenine-Induced Nephrotoxicity MA Saleh,Pharmaceuticals (Basel),2020
- Biological Effects of a Three-Dimensionally Printed Ti6Al4V Scaffold Coated with Piezoelectric BaTiO3 Nanoparticles on Bone Formation W Liu,ACS Applied Materials & Interfaces,2020
- Reduced Platelet miR-223 Induction in Kawasaki Disease Leads To Severe Coronary Artery Pathology Through a miR-223/PDGFRβ Vascular Smooth Muscle Cell Axis Yuan Zhang,CIRCULATION RESEARCH,2020
- Low temperature plasma induces angiogenic growth factor via up-regulating hypoxia?inducible factor 1α in human dermal fibroblasts Hui SongCui.et al,Archives of Biochemistry and Biophysics,2017
产品评价
相关产品
靶点详情
-
最新研究进展：PDGF-BB是血小板源性生长因子（platelet-derived growth factor，PDGF）家族的一个亚型，由PDGF-B两个亚基组成。最近的研究表明，PDGF-BB参与了多种生物学过程，如细胞增殖、分化、迁移和细胞外基质合成等。此外，PDGF-BB在多种疾病中也发挥着重要作用，如肿瘤、动脉粥样硬化和组织损伤等。因此，PDGF-BB成为了治疗靶点的研究热点。一些研究已经探索了PDGF-BB的抑制剂和抗体，以期望发现更有效的治疗方案。
