Human Protein Wnt-7b(WNT7B) ELISA kit
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中文名称：人Wnt-7b蛋白(WNT7B)酶联免疫试剂盒
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货号：CSB-EL026142HU
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规格：96T/48T
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价格：￥3600/￥2500
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其他：
产品详情
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产品描述：人Wnt-7b蛋白(WNT7B)酶联免疫试剂盒（CSB-EL026142HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆,组织匀浆样本中的WNT7B含量。WNT7B 是重要靶点。它参与 Wnt 信号通路，在胚胎发育、细胞增殖与分化等方面发挥关键作用。研究其机制有助于揭示疾病发生发展原理，为相关疾病如癌症等的诊断、治疗及药物研发提供新方向与潜在靶点。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于基础研究领域，如探究Wnt信号通路在组织修复、干细胞分化或疾病模型中的作用机制，尤其适合需要从复杂生物样本（如炎症相关组织匀浆或循环系统样本）中定量分析WNT7B表达变化的实验需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
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别名：Protein Wnt-7b ELISA Kit; Wingless related MMTV integration site 7B ELISA Kit; Wingless type MMTV integration site family member 7B ELISA Kit; WNT ELISA Kit; WNT7B ELISA Kit; WNT7B_HUMAN ELISA Kit
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缩写：
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Uniprot No.：
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种属：Homo sapiens (Human)
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样本类型：serum, plasma,tissue homogenates
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检测范围：0.156 ng/mL-10 ng/mL
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灵敏度：0.039 ng/mL
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Stem Cells
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测定原理：quantitative
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测定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
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线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human WNT7B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
102
Range %
95-108
1:2
Average %
96
Range %
91-100
1:4
Average %
87
Range %
82-93
1:8
Average %
95
Range %
88-102
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回收率：
The recovery of human WNT7B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
101
96-106
EDTA plasma (n=4)
94
90-99
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标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml
OD1
OD2
Average
Corrected
10
2.532
2.634
2.583
2.444
5
1.683
1.767
1.725
1.586
2.5
0.901
0.928
0.915
0.775
1.25
0.554
0.579
0.567
0.427
0.625
0.363
0.349
0.356
0.217
0.312
0.272
0.259
0.265
0.126
0.156
0.189
0.194
0.191
0.052
0
0.143
0.136
0.139
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数据处理：
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货期：3-5 working days
产品评价
相关产品
靶点详情
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功能：Ligand for members of the frizzled family of seven transmembrane receptors that functions in the canonical Wnt/beta-catenin signaling pathway. Required for normal fusion of the chorion and the allantois during placenta development. Required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation.
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基因功能参考文献：
- We identified a novel genome-wide significant association rs10453441 in the WNT7B gene on chromosome 22 as a novel SNP for central corneal thickness in Latinos. PMID: 28171582
- the present study detected abnormal upregulation in the levels of Wnt2b and Wnt7b, and hypothesized that the alterations may be due to the ectopic opening of chromatin structure. PMID: 26548512
- WNT7B as a novel susceptibility gene for axial length and corneal curvature and is associated with myopia and extreme myopia. PMID: 25823570
- Results illustrated the critical role of myeloid WNT7B in tumor progression, acting at the levels of angiogenesis, invasion, and metastasis. PMID: 24638982
- Results suggest that AR-regulated WNT7B signaling is critical for the growth of CRPC and development of the osteoblastic bone response characteristic of advanced prostate cancer. PMID: 23386686
- WNT7B can serve as a primary determinant of differential Wnt/beta-catenin activation in pancreatic adenocarcinoma. PMID: 23416978
- Wnt7B was expressed at high concentrations in regions of active hyperplasia, metaplasia, and fibrotic change in idiopathic pulmonary fibrosis patients. PMID: 22838404
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亚细胞定位：Secreted, extracellular space, extracellular matrix. Secreted.
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蛋白家族：Wnt family
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组织特异性：Moderately expressed in fetal brain, weakly expressed in fetal lung and kidney, and faintly expressed in adult brain, lung and prostate.
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数据库链接：
