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  4. Human Protein disulfide-isomerase A4(PDIA4) ELISA kit

Human Protein disulfide-isomerase A4(PDIA4) ELISA kit

说明书下载
  • 中文名称：
    人蛋白质二硫键异构酶A4(PDIA4)酶联免疫试剂盒
  • 货号：
    CSB-EL017722HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    人蛋白质二硫键异构酶A4(PDIA4)酶联免疫试剂盒（CSB-EL017722HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的PDIA4含量。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，本产品适用于基础科研中PDIA4相关分子机制探索，包括但不限于内质网应激模型构建、氧化还原信号通路研究以及疾病相关生物标志物筛选等实验场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Calcium binding protein intestinal related ELISA Kit; Endoplasmic reticulum resident protein 70 ELISA Kit; Endoplasmic reticulum resident protein 72 ELISA Kit; EPR70 ELISA Kit; EPR72 ELISA Kit; ER protein 70 ELISA Kit; ER protein 72 ELISA Kit; ERP 70 ELISA Kit; ERp 72 ELISA Kit; ERp-72 ELISA Kit; ERp70 ELISA Kit; ERp72 ELISA Kit; PDIA 4 ELISA Kit; Pdia4 ELISA Kit; PDIA4_HUMAN ELISA Kit; PDIR ELISA Kit; Protein disulfide isomerase A4 ELISA Kit; Protein disulfide isomerase associated 4 ELISA Kit; Protein disulfide isomerase family A member 4 ELISA Kit; protein disulfide isomerase related protein (calcium-binding protein; intestinal-related) ELISA Kit; Protein disulfide isomerase related protein ELISA Kit; Protein disulfide-isomerase A4 ELISA Kit; Protein ERp 72 ELISA Kit; Protein ERp72 ELISA Kit
  • 缩写：
    PDIA4
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    23.5 pg/ml-1500 pg/ml
  • 灵敏度：
    5.8 pg/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human PDIA4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 94  
    Range % 89-97  
    1:2 Average % 100  
    Range % 96-104  
    1:4 Average % 90  
    Range % 88-93  
    1:8 Average % 91  
    Range % 86-94  
  • 回收率：
    The recovery of human PDIA4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 91 87-94  
    EDTA plasma (n=4) 104 99-106  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    1500 1.960 1.977 1.969 1.817  
    750 1.284 1.300 1.292 1.140  
    375 0.725 0.713 0.719 0.567  
    187.5 0.412 0.406 0.409 0.257  
    94 0.287 0.277 0.282 0.130  
    47 0.220 0.215 0.218 0.066  
    23.5 0.184 0.178 0.181 0.029  
    0 0.150 0.154 0.152    
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

产品评价

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PDIA4 antibodies

PDIA4 Proteins

靶点详情

  • 基因功能参考文献：
    1. up-regulated by IL11 in first trimester placenta PMID: 28487027
    2. PDIA4 as a negative regulator of cancer cell apoptosis. PMID: 28534513
    3. Participants with metabolic syndrome (MetS) had significantly higher serum PDIA4 levels than those without MetS (P<0.001). After adjustments, the individuals with the highest PDIA4 tertile were associated with a higher risk of MetS than those with the lowest tertile (OR = 4.83, 95% CI: 2.71-8.60). PMID: 28650993
    4. CDDP-resistant non-small lung cancer cells undergo profound remodeling of their endoplasmic reticulum (ER) proteome (>80 proteins identified by proteomics) and exhibit a dramatic overexpression of two protein disulfide isomerases, PDIA4 and PDIA6. PMID: 24464223
    5. novel esophageal squamous cell carcinoma marker PDIA4 was identified by mass spectrometry and immunohistochemical analysis PMID: 21743296
    6. Nox1 associates with ERp72, which involves its N-terminus encompassing a Ca(2+)-binding site and the first TRX-like motif. PMID: 18620548

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  • 亚细胞定位：
    Endoplasmic reticulum lumen. Melanosome.
  • 蛋白家族：
    Protein disulfide isomerase family
  • 数据库链接：

    HGNC: 30167

    KEGG: hsa:9601

    STRING: 9606.ENSP00000286091

    UniGene: Hs.93659

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