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  4. Human SLAM family member 7(SLAMF7) ELISA kit

Human SLAM family member 7(SLAMF7) ELISA kit

说明书下载
  • 中文名称：
    人SLAM家族成员7(SLAMF7)酶联免疫试剂盒
  • 货号：
    CSB-EL021369HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：
    CUSABIO人SLAM家族成员7（SLAMF7）酶联免疫试剂盒（货号：CSB-EL021369HU），基于双抗体夹心法原理设计，适用于血清、血浆及组织匀浆样本中SLAMF7蛋白的定量检测。SLAMF7作为免疫调节相关分子，在自然杀伤细胞（NK细胞）和特定淋巴细胞表面表达，参与细胞间信号传导及免疫应答调控，是研究免疫相关疾病机制的重要靶点。试剂盒检测灵敏度为78 pg/mL，线性检测范围为78-5000 pg/mL，可精准检测低丰度样本，满足科研场景中不同浓度样本的分析需求。实验操作采用预包被特异性抗体的96孔板，通过标准品浓度梯度建立标准曲线，实现目标蛋白的绝对定量分析。该产品适用于基础研究领域，如肿瘤免疫微环境、自身免疫性疾病或感染性疾病模型中SLAMF7的表达水平检测，为探索其生物学功能及病理机制提供可靠工具。试剂盒包含必要实验组分，需配合酶标仪进行吸光度测定，建议严格按照说明书进行样本预处理和实验操作以确保数据准确性。
  • 别名：
    SLAMF7 ELISA Kit; CS1 ELISA Kit; UNQ576/PRO1138 ELISA Kit; SLAM family member 7 ELISA Kit; CD2 subset 1 ELISA Kit; CD2-like receptor-activating cytotoxic cells ELISA Kit; CRACC ELISA Kit; Membrane protein FOAP-12 ELISA Kit; Novel Ly9 ELISA Kit; Protein 19A ELISA Kit; CD antigen CD319 ELISA Kit
  • 缩写：
    SLAMF7
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    78 pg/mL-5000 pg/mL
  • 灵敏度：
    19.5 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human SLAMF7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:5Average %106
    Range %100-112
    1:10Average %98
    Range %84-102
    1:20Average %84
    Range %80-88
    1:40Average %95
    Range %90-100
  • 回收率：
    The recovery of human SLAMF7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9389-98
    EDTA plasma (n=4)9994-110
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1OD2AverageCorrected
    50002.396 2.501 2.449 2.272
    25001.907 1.824 1.866 1.689
    12501.295 1.234 1.265 1.088
    6250.783 0.743 0.763 0.586
    3120.462 0.448 0.455 0.278
    1560.365 0.342 0.354 0.177
    780.259 0.247 0.253 0.076
    00.174 0.180 0.177
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

产品评价

相关产品

SLAMF7 antibodies

SLAMF7 Proteins

相关问答

■ 常见问题解答
Q:

Per customers request, please provide the following information regarding Human SLAM family member 7, SLAMF7 ELISA Kit CSB-EL021369HU
1)The active ingredient of the stop solution and its concentration ?
2)The type, concentration, and state (liquid/lyophilized) of the preservatives used ?
3) If the components are lyophilized, what is the concentration prior to reconstitution (i.e. mass of preservative used/ total mass of lyophilized vial contents)?
4)Expression host of the standards ?
5)Which components have H202 with the concentration of H202 in each, if any?
6) Are there any components that contain hydrogen peroxide & Urea hydrogen peroxide (Carbamide peroxide) in any of the components in this kit?
7) If yes, which components contain hydrogen peroxide & Urea hydrogen peroxide (Carbamide peroxide) and the concentration?
Will the information always be the same for all of your competitive kits ? what about your sandwich kits?

A:
Thanks for your inquiry!
CSB-EL021369HU
Stop solution is 4N sulfuric acid.
Standard is NS0-derived recombinant protein. It is lyophilized powder. The concentration after reconsititution is 5000pg/mL. The preservative is liquid proclin 300(1:3000).
This kit doesn't contain hydrogen peroxide & Urea hydrogen peroxide (Carbamide peroxide).
This information is not the same for all our kits. It is better to send us inquiry for a specific kit.
Pls let me know if you have any further questions. Thank you.

靶点详情

  • 功能：
    Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Isoform 1 mediates NK cell activation through a SH2D1A-independent extracellular signal-regulated ERK-mediated pathway. Positively regulates NK cell functions by a mechanism dependent on phosphorylated SH2D1B. Downstream signaling implicates PLCG1, PLCG2 and PI3K. In addition to heterotypic NK cells-target cells interactions also homotypic interactions between NK cells may contribute to activation. However, in the absence of SH2D1B, inhibits NK cell function. Acts also inhibitory in T-cells. May play a role in lymphocyte adhesion. In LPS-activated monocytes negatively regulates production of proinflammatory cytokines.; Isoform 3 does not mediate any NK cell activation.
  • 基因功能参考文献：
    1. Memory CD8+ T cells from SLE patients displayed decreased amounts of SLAMF7, a surface receptor that characterizes effector CD8+ T cells. Ligation of SLAMF7 increased CD8+ T cell degranulation capacity and the percentage of IFNgamma-producing cells in response to antigen challenge in SLE patients and healthy controls. SLAMF7 engagement promoted cytotoxic lysis of target cells in response to stimulation with viral antig... PMID: 28076903
    2. phagocytosis of haematopoietic tumour cells during SIRPalpha-CD47 blockade was strictly dependent on SLAM family receptors in vitro and in vivo; in both mouse and human cells, this function required a single SLAM family member, SLAMF7 (also known as CRACC, CS1, CD319), expressed on macrophages and tumour cell targets PMID: 28424516
    3. Cohort statistics revealed a significant increase of circulating sSLAMF7 in multiple myeloma patients versus normal controls PMID: 27116021
    4. Blimp-1 regulates the transcription of CS1 gene in NK and B cell lines from multiple myeloma and diffuse large B cell lymphoma patients. PMID: 26310579
    5. SLAMF7-triggered inhibition is mediated by a mechanism involving Src kinases, CD45, and SHIP-1 that is defective in MM cells PMID: 25312647
    6. Our data highlight the therapeutic potential of targeting CD319 in rheumatoid arthritis PMID: 24299175
    7. SLAMF7 plays an inhibitory role in human monocytes to control proinflammatory immune responses. PMID: 23695528
    8. These results suggest a role for CD319 and CD229 in the systemic lupus erythematosus disease process. PMID: 23956418
    9. These data suggest an involvement of CRACC-mediated NK cell activation in periodontal tissue destruction and point to a plausible distinction in the pathobiology of aggressive and chronic periodontitis. PMID: 23250953
    10. CS1-L and CS1-S may differentially regulate human NK cell functions PMID: 15368295
    11. CS1 may play a role in the regulation of B lymphocyte proliferation during immune responses PMID: 17878365
    12. CS1 was expressed at adhesion-promoting uropod membranes of polarized Multiple Myeloma cells, andis required for MM cell adhesion to bone marrow stromal cells PMID: 17906076
    13. HuLuc63 eliminates myeloma cells, at least in part, via NK-mediated ADCC and shows the therapeutic potential of targeting CS1 with HuLuc63 for the treatment of multiple myeloma. PMID: 18451245

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  • 亚细胞定位：
    Membrane; Single-pass type I membrane protein.
  • 组织特异性：
    Expressed in spleen, lymph node, peripheral blood leukocytes, bone marrow, small intestine, stomach, appendix, lung and trachea. Expression was detected in NK cells, activated B-cells, NK-cell line but not in promyelocytic, B-, or T-cell lines. Expressed
  • 数据库链接：

    HGNC: 21394

    OMIM: 606625

    KEGG: hsa:57823

    STRING: 9606.ENSP00000357022

    UniGene: Hs.517265

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