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  4. Human Spondin-1(SPON1) ELISA kit

Human Spondin-1(SPON1) ELISA kit

文献引用 (4) 说明书下载
  • 中文名称：
    人脊椎蛋白-1(SPON1)酶联免疫试剂盒
  • 货号：
    CSB-EL022599HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    人脊椎蛋白-1(SPON1)酶联免疫试剂盒（CSB-EL022599HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SPON1含量。SPON1（脊椎蛋白-1）是一种细胞外基质结构成分，参与细胞粘附和蛋白质结合活性的调节。其研究机制涉及在细胞外空间与胶原蛋白共定位，并可能参与淀粉样蛋白前体蛋白的分解代谢过程。在疾病如阿尔茨海默病中，SPON1的表达和功能可能发生变化，影响疾病的发病机制。试剂盒检测范围为0.156 ng/mL-10 ng/mL，科研人员可通过探究SPON1在神经退行性疾病模型中的表达特征，分析其在肿瘤微环境中的调控作用，或评估骨骼发育相关疾病中该蛋白的生物标志物价值；适用于体外实验中的基础机制研究、疾病模型分析及生物标志物筛选等科研场景，为探索SPON1的分子功能及其在病理生理过程中的作用提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    SPON1 ELISA kit; KIAA0762 ELISA kit; VSGPSpondin-1 ELISA kit; F-spondin ELISA kit; Vascular smooth muscle cell growth-promoting factor ELISA kit
  • 缩写：
    SPON1
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human spondin-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %84
    Range %80-91
    1:2Average %86
    Range %81-92
    1:4Average %92
    Range %87-100
    1:8Average %95
    Range %88-101
  • 回收率：
    The recovery of human spondin-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9790-104
    EDTA plasma (n=4)9386-97
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.504 2.463 2.484 2.365
    52.006 2.042 2.024 1.905
    2.51.506 1.479 1.493 1.374
    1.250.973 0.897 0.935 0.816
    0.6250.511 0.518 0.515 0.396
    0.3120.285 0.268 0.276 0.157
    0.1560.176 0.187 0.182 0.063
    00.121 0.117 0.119 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

产品评价

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SPON1 antibodies

SPON1 Proteins

靶点详情

  • 功能：
    Cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro. May contribute to the growth and guidance of axons in both the spinal cord and the PNS. Major factor for vascular smooth muscle cell.
  • 基因功能参考文献：
    1. gene expression experiment revealed that CYP2B6, SPON1, and GSG1L can be activated concomitantly through a constitutive androstane receptor (CAR) activation pathway PMID: 27010727
    2. SPON1 promotes metastatic progression through Fak and Src dependent pathway in human osteosarcoma. PMID: 26032498
    3. These data suggest that SPON1 may be associated with the differential rate of cognitive decline in Alzheimer disease. PMID: 23535033
    4. High SPON1 expression is associated with high-grade gliomas. PMID: 24158112
    5. Genome-wide scan of healthy human connectome discovers SPON1 gene variant influencing dementia severity PMID: 23471985
    6. downregulates osteoclast recruitment to the root side of periodontal tissue via low-density lipoprotein receptor-related protein 8 and inhibits differentiation of osteoclastic precursors PMID: 21488757
    7. These findings indicate that F-spondin regulates the differentiation of human cementoblast-like cells via BMP7 expression. PMID: 22244873
    8. The unique feature of F-spondin FS domain is the presence of three disulfide bonds associated with the N- and C-termini of the domain and a highly conserved N-linked glycosylation site. PMID: 21569239
    9. expression profile of VSGP/F-spondin identifies this molecule as a potential diagnostic marker or target for developing therapeutic strategies to treat ovarian carcinoma PMID: 16103746
    10. identified F-spondin as a promoting factor for cementoblastic differentiation PMID: 16965763
    11. The crystal structure of the reelin-N domain resolved to 2.0 A reveals a variant immunoglobulin-like fold and potential heparin-binding sites. PMID: 18602404
    12. These studies identify F-spondin as a novel protein in osteoarthritis cartilage, where it may act in situ at lesional areas to activate latent TGF-beta and induce cartilage degradation. PMID: 18780763

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  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 组织特异性：
    Highest expression in lung, lower expression in brain, heart, kidney, liver and testis, and lowest expression in pancreas, skeletal muscle and ovary. Not expressed in spleen.
  • 数据库链接：

    HGNC: 11252

    OMIM: 604989

    KEGG: hsa:10418

    UniGene: Hs.643864

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